Description

3H-Diazirine,3-Fluoro-3-(2,2,2-Trifluoroethoxy)-(9Ci) is a specialized diazirine-based photoaffinity labeling reagent, a critical tool for advanced biochemical and pharmaceutical research. Its core value lies in its ability to form highly reactive carbene intermediates upon photolysis, enabling the covalent cross-linking and identification of biomolecular interaction sites. This compound is essential for researchers in medicinal chemistry, chemical biology, and proteomics who require precise molecular probes for target identification and drug discovery studies. The product is supplied with a guaranteed Certificate of Analysis to ensure batch-to-batch consistency for your critical applications.

Application

• Photoaffinity Labeling (PAL): A key reagent for covalently tagging proteins, nucleic acids, or other biological targets upon UV light activation to study binding sites and molecular interactions.
• Proteomics & Target Identification: Used to identify unknown protein targets of bioactive small molecules or drugs in complex biological systems.
• Medicinal Chemistry & Drug Discovery: Integral for mapping ligand-receptor interactions and validating drug candidate mechanisms of action.
• Chemical Biology Probes: Serves as a versatile building block for synthesizing bifunctional probes that combine a photo-crosslinker with a reporter tag (e.g., biotin, fluorophore).
• Bioconjugation & Material Science: Enables the surface functionalization of materials with biological molecules for biosensor development and biomaterial engineering.
• Structural Biology Studies: Assists in probing protein structure and dynamics by introducing cross-links that can be analyzed via mass spectrometry.

Basic Information

Product Name	3H-Diazirine,3-Fluoro-3-(2,2,2-Trifluoroethoxy)-(9Ci)
CAS No.	154914-84-8
Molecular Formula	C4H4F4N2O
Molecular Weight	172.08 g/mol
Synonyms	3-Fluoro-3-(2,2,2-trifluoroethoxy)diazirine; 3-(2,2,2-Trifluoroethoxy)-3-fluorodiazirine; 3-Fluoro-3-trifluoroethoxydiazirine; Diazirine, 3-fluoro-3-(2,2,2-trifluoroethoxy)-; Photoaffinity Labeling Reagent; PAL Reagent; Trifluoroethoxyfluorodiazirine
EINECS	Contact for details

Quality Control

Every batch of our 3H-Diazirine,3-Fluoro-3-(2,2,2-Trifluoroethoxy)-(9Ci) is synthesized and purified under controlled conditions to meet the stringent requirements of research and development. Our products undergo rigorous quality testing, including HPLC, NMR, and MS analysis, to ensure high purity and structural identity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment to guarantee traceability and support your regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated place at recommended temperatures between -20°C to -10°C for long-term stability, or at 2-8°C for short-term use. Due to its highly volatile and light-sensitive nature, allow the container to reach ambient temperature before opening to minimize moisture ingress and degradation. Keep away from heat, sparks, and open flame.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 97%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.