Description

2-Methoxy-4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Benzoic Acid, Methyl Ester is a specialized photolabile crosslinking agent, with CAS number 154187-50-5, designed for advanced photoaffinity labeling studies. Its core value lies in the highly reactive carbene generated upon UV irradiation, enabling the covalent capture of transient biomolecular interactions. This compound is essential for researchers and developers in pharmaceutical discovery, chemical biology, and proteomics who require precise tools for target identification and binding site mapping.

Application

• Photoaffinity Labeling (PAL): Primary application for covalently tagging drug targets, receptors, and enzymes to study binding sites and protein-ligand interactions.
• Proteomics Research: Used as a chemical probe to identify and characterize unknown protein targets and signaling pathways.
• Drug Discovery & Development: Integral in early-stage discovery for validating drug-target engagement and elucidating mechanisms of action.
• Chemical Biology Tool: Employed in the synthesis of bioconjugates and activity-based probes for functional studies in live cells.
• Material Science: Potential use in surface functionalization and creating light-responsive polymeric materials.

Basic Information

Item	Details
Product Name	2-Methoxy-4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Benzoic Acid, Methyl Ester
CAS No.	154187-50-5
Molecular Formula	C12H9F3N2O3
Molecular Weight	286.21 g/mol
Synonyms	4-[3-(Trifluoromethyl)-3H-diazirin-3-yl]-2-methoxybenzoic acid methyl ester; Methyl 2-methoxy-4-[3-(trifluoromethyl)diazirin-3-yl]benzoate; Methyl 4-(3-(trifluoromethyl)-3H-diazirin-3-yl)-2-methoxybenzoate; Trifluoromethylphenyldiazirine benzoic acid methyl ester; Photoaffinity labeling reagent; PAL reagent; Carbene precursor; Diazirine crosslinker.
EINECS	Contact for details

Quality Control

Every batch of our 2-Methoxy-4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Benzoic Acid, Methyl Ester is manufactured and tested under strict quality protocols to ensure high purity and performance consistency for research applications. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and impurity profiles, typically assessed via HPLC, NMR, and MS. Our quality system is designed to meet the stringent requirements of research and development laboratories worldwide.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) for long-term stability. This compound is strictly light-sensitive; all handling and aliquoting should be performed under subdued light or using amber glassware to prevent premature decomposition. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Melting Point	Contact for details
Water Content (KF)	< 0.5%
Solubility	Soluble in common organic solvents (DMSO, DMF, Acetonitrile)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.