Description

Man(9)(Glcnac)(2)-Diphosphate-Dolichol is a complex, high-purity lipid-linked oligosaccharide (LLO) intermediate, critical for N-linked protein glycosylation pathways. This compound serves as the essential donor substrate for the oligosaccharyltransferase (OST) complex, enabling the transfer of the glycan precursor (Glc3Man9GlcNAc2) to nascent polypeptides in the endoplasmic reticulum. It is an indispensable research-grade biochemical for scientists in glycobiology, enzymology, and pharmaceutical development, particularly for studying congenital disorders of glycosylation (CDGs) and developing glycosylation-based therapeutics.

Application

• Biochemical Research: Essential substrate for in vitro studies of the oligosaccharyltransferase (OST) enzyme mechanism and kinetics.
• Glycobiology Studies: Used to investigate the fundamental processes of N-linked glycosylation, protein folding, and quality control in the ER.
• Enzyme Activity Assays: Serves as a critical reagent in high-throughput screening assays to identify modulators or inhibitors of glycosylation enzymes.
• Disease Modeling: Key tool for researching Congenital Disorders of Glycosylation (CDGs) related to dolichol-phosphate metabolism and LLO assembly.
• Pharmaceutical Development: Used in the development and testing of novel therapeutic agents targeting aberrant glycosylation in cancer and other diseases.
• Reference Standard: Acts as a high-purity analytical standard for the identification and quantification of LLO intermediates in complex biological samples via LC-MS or other techniques.

Basic Information

Product Name	Man(9)(Glcnac)(2)-Diphosphate-Dolichol
CAS No.	143709-90-4
Molecular Formula	C?H?N2O?P2 (Complex structure, contact for details)
Molecular Weight	Contact for details
Synonyms	Dolichyl Diphosphate N-Acetylglucosaminyl(2) Mannosyl(9); Dolichyl-PP-(GlcNAc)2(Man)9; LLO (Lipid-Linked Oligosaccharide) precursor; GlcNAc2Man9-PP-Dolichol; Dol-PP-GlcNAc2Man9; N-Glycan Precursor (Man9GlcNAc2-PP-Dol); Oligosaccharyl Diphosphodolichol; Dolichol-linked oligosaccharide (Man9GlcNAc2).
EINECS	Contact for details

Quality Control

Our Man(9)(Glcnac)(2)-Diphosphate-Dolichol is produced and handled under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot is accompanied by comprehensive analytical data, including purity assessment via HPLC and structural confirmation by mass spectrometry. Certificates of Analysis (COA) detailing identity, purity, and stability are available upon request to support your critical research and development work.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, solutions should be prepared in anhydrous solvents and kept at -80°C. Allow the vial to reach room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid or film
Identification (MS)	Complies (Exact mass confirmation)
Purity (HPLC)	≥ 95% (Area %)
Biological Activity	Functional as OST substrate (Contact for assay details)
Solubility	Soluble in chloroform/methanol/water mixtures, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.