Description

5-Azaprotoporphyrin Dimethyl Ester is a synthetic porphyrin analog where a nitrogen atom replaces a methine bridge in the protoporphyrin IX core. This structural modification is crucial for its role as a potent and selective inhibitor of heme oxygenase, an enzyme central to heme catabolism and cellular stress response. It is an essential biochemical research tool for scientists in pharmacology, toxicology, and biochemistry studying oxidative stress, cytoprotection, and iron metabolism pathways.

Application

• Heme Oxygenase (HO) Inhibition Studies: Primary research application for investigating the HO-1 and HO-2 isozymes in models of inflammation, ischemia-reperfusion injury, and neurodegenerative diseases.
• Biochemical Pathway Analysis: Used to probe the biological roles of carbon monoxide, bilirubin, and free iron released during heme degradation.
• Pharmaceutical Research: Serves as a reference compound and a starting point for the development of novel therapeutic agents targeting the heme oxygenase pathway.
• Cell Culture & In Vitro Assays: Applied in cell-based experiments to modulate cellular redox state and study cytoprotective mechanisms.
• Enzyme Kinetics & Mechanism Studies: Utilized to determine inhibitory constants (Ki, IC50) and elucidate the interaction mechanism with heme oxygenase enzymes.
• Reference Standard: Acts as a high-purity analytical standard for method development and validation in chromatographic analysis (HPLC, LC-MS) of porphyrin derivatives.

Basic Information

Product Name	5-Azaprotoporphyrin Dimethyl Ester
CAS No.	141622-84-6
Molecular Formula	C₃₆H₃₈N₆O₄
Molecular Weight	618.73 g/mol
Synonyms	5-Azaprotoporphyrin IX Dimethyl Ester; Azaprotoporphyrin Dimethyl Ester; 5-Aza-protoporphyrin IX dimethyl ester; Protoporphyrin IX, 5-aza-, dimethyl ester; Heme Oxygenase Inhibitor (Zn(II)-5-Azaprotoporphyrin IX Dimethyl Ester precursor); 5-AZA-PPIX-DME
EINECS	Contact for details

Quality Control

Our 5-Azaprotoporphyrin Dimethyl Ester is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure high purity and batch-to-batch consistency, critical for reliable research outcomes. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We support research compliance with traceable documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark purple to brown solid
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Related Substances (HPLC)	Individual impurity ≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in chloroform, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.