Description

Succinimidyl 4-Hydrazinobenzoate is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a hydrazine group. This compound is critical for creating stable conjugates, particularly for labeling biomolecules like antibodies and proteins with reporter molecules or solid supports. It is essential for researchers and manufacturers in the fields of bioconjugation, diagnostics, and pharmaceutical development who require reliable, site-specific coupling chemistry.

Application

• Bioconjugation and Protein Labeling: Site-specific coupling of antibodies, proteins, or peptides with fluorophores, enzymes, or other probes via the hydrazine moiety.
• Diagnostic Assay Development: Key reagent for preparing enzyme-linked immunosorbent assay (ELISA) components and other immunoassay conjugates.
• Affinity Chromatography Ligand Immobilization: Covalent attachment of biomolecules to agarose or other resin surfaces for purification columns.
• Drug Delivery System Fabrication: Used in the synthesis of antibody-drug conjugates (ADCs) and other targeted therapeutic constructs.
• Surface Modification: Functionalization of biosensor chips, microarrays, and nanoparticles for enhanced biomolecule capture.
• Carbohydrate and Glycoprotein Labeling: Targeting oxidized sugar residues (aldehydes) on glycans for analysis or detection.

Basic Information

Product Name	Succinimidyl 4-Hydrazinobenzoate
CAS No.	133081-23-9
Molecular Formula	C11H11N3O4
Molecular Weight	249.22 g/mol
Synonyms	4-Hydrazinobenzoic Acid N-Hydroxysuccinimide Ester; 4-(N-Succinimidyloxycarbonyl)phenylhydrazine; SHBH; NHS-Hydrazide; 4-Hydrazinylbenzoic Acid 2,5-Dioxopyrrolidin-1-yl Ester; p-Hydrazinobenzoic Acid Succinimidyl Ester; 4-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)carbonyl]phenylhydrazine
EINECS	Contact for details

Quality Control

Our Succinimidyl 4-Hydrazinobenzoate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for sensitive bioconjugation work. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 97%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO; Slightly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.