Description

4-(2-n-Maleimido)Methyl Benzohydrazide-Hcl is a specialized heterobifunctional crosslinking agent featuring both a maleimide and a hydrazide functional group. This compound is critical for creating stable, site-specific conjugations between biomolecules, such as antibodies and enzymes, or between biomolecules and surfaces. It is primarily utilized by researchers and manufacturers in the fields of bioconjugation, diagnostics development, and advanced pharmaceutical research to develop targeted therapeutics and sensitive assay systems.

Application

• Bioconjugation Reagent: For the covalent coupling of peptides, proteins, or antibodies to other molecules, nanoparticles, or solid supports.
• Diagnostic Kit Development: Used in the manufacture of enzyme-linked immunosorbent assays (ELISA) and other immunoassay components.
• Antibody-Drug Conjugate (ADC) Research: Serves as a linker molecule in the development of targeted cancer therapies.
• Surface Functionalization: For modifying biosensor chips, microarray slides, or chromatographic media with specific biomolecular capture agents.
• Protein Labeling and Tagging: Enables the attachment of fluorescent dyes, affinity tags, or other probes to proteins for tracking and purification.
• Polymer Chemistry: Acts as a reactive monomer or crosslinker in the synthesis of functionalized polymers for biomedical applications.

Basic Information

Product Name	4-(2-n-Maleimido)Methyl Benzohydrazide-Hcl
CAS No.	129506-88-3
Molecular Formula	C13H14N4O3 • HCl
Molecular Weight	310.73 g/mol (Free base: 274.28 g/mol)
Synonyms	4-(2-Maleimidomethyl)benzohydrazide hydrochloride; N-(4-Hydrazinylcarbonylbenzyl)maleimide hydrochloride; 4-(Maleimidomethyl)benzoic acid hydrazide hydrochloride; Maleimidomethylbenzoyl hydrazide hydrochloride; MHBH-HCl; Benzohydrazide, 4-[(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)methyl]-, monohydrochloride; 1-(4-Hydrazinocarbonylbenzyl)pyrrole-2,5-dione hydrochloride
EINECS	Contact for details

Quality Control

Our 4-(2-n-Maleimido)Methyl Benzohydrazide-Hcl is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and residual solvent analysis, to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive); keep the container tightly sealed in a desiccated environment to prevent degradation. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.