Description

2-Methyl-6-(5-Acetamido-3,5-Dideoxy-α-Glycero-Galacto-Nonulopyranosylonic Acid)Xanthene-9-Spiro-1'-Isobenzofuran-3'-One is a sophisticated, high-purity fluorogenic substrate and chemical intermediate designed for advanced biochemical research and diagnostic applications. Its core value lies in its specific reactivity, which enables highly sensitive detection and quantification of enzymatic activity, particularly in complex biological matrices. This compound is essential for researchers and manufacturers in the fields of molecular diagnostics, enzyme assay development, and pharmaceutical research, where precision and reliability are paramount.

Application

• Fluorogenic Substrate for Enzymatic Assays: Primarily used as a specialized substrate for detecting and quantifying the activity of specific enzymes, such as neuraminidases or glycosidases, in research and diagnostic kits.
• Molecular Diagnostics: A key component in the development of sensitive diagnostic assays for viral infections (e.g., influenza) and other diseases where specific enzyme markers are relevant.
• Biochemical Research: Employed in academic and industrial research laboratories to study enzyme kinetics, inhibition, and mechanism of action.
• Pharmaceutical Development: Used in high-throughput screening (HTS) platforms to identify and characterize potential therapeutic enzyme inhibitors.
• Proteomics and Glycobiology Studies: Serves as a critical tool for investigating glycosylation patterns and carbohydrate-processing enzymes.
• IVD (In-Vitro Diagnostic) Manufacturing: Integrated into the production of commercial diagnostic reagents and kits that require high specificity and low background signal.

Basic Information

Product Name	2-Methyl-6-(5-Acetamido-3,5-Dideoxy-α-Glycero-Galacto-Nonulopyranosylonic Acid)Xanthene-9-Spiro-1'-Isobenzofuran-3'-One
CAS No.	128683-43-2
Molecular Formula	C₃₀H₃₀N₂O₁₂
Molecular Weight	610.57 g/mol
Synonyms	4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid; 4-MU-α-Neu5Ac; 4-Methylumbelliferyl-α-N-acetylneuraminic acid; MUNANA; 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid; Fluorogenic neuraminidase substrate; α-(4-Methylumbelliferyl)-N-acetylneuraminic acid; 4-MU-α-D-Neu5Ac
EINECS	Contact for details

Quality Control

Our production of this fluorogenic substrate adheres to stringent quality protocols to ensure batch-to-batch consistency and performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification, NMR and MS for structural confirmation, and functional activity testing in standardized enzymatic assays. We provide detailed Certificates of Analysis (COA) with every shipment, documenting critical parameters such as purity, water content, and specific activity. Our quality system is designed to meet the exacting requirements of research and diagnostic manufacturing.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. Under these conditions, the product is stable for at least 24 months. The compound is hygroscopic and light-sensitive; allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Specific Optical Rotation	Contact for details
Functional Activity (Enzymatic Assay)	≥ 95% of reference standard
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.