Description

Mannosyl(5)-n-Acetyl(2)-Glucose Diphosphate Dolichol CAS NO 119913-05-2 is a critical, high-purity biochemical intermediate essential for the biosynthesis of N-linked glycoproteins. This compound serves as a key activated sugar donor in the oligosaccharyltransferase (OST) complex, facilitating the transfer of the glycan precursor onto asparagine residues of nascent polypeptides. It is therefore indispensable for researchers and manufacturers in pharmaceutical development, enzymology studies, and the production of complex biologics where proper protein glycosylation is vital for function, stability, and efficacy.

Application

• Biochemical Research & Enzymology: As a substrate for studying the oligosaccharyltransferase (OST) enzyme complex and the N-glycosylation pathway in vitro.
• Pharmaceutical Development: Used in the research and development of therapeutics targeting glycosylation disorders, congenital disorders of glycosylation (CDGs), and certain viral infections.
• Glycobiology Studies: A crucial reagent for investigating the structure, biosynthesis, and function of N-linked glycans in cell biology and disease models.
• Production of Reference Standards: Serves as a high-purity standard for analytical methods (HPLC, MS) used in quality control of glycoprotein-based biologics.
• Cell-Free Protein Synthesis Systems: Incorporated into in vitro translation/glycosylation systems to produce correctly glycosylated recombinant proteins.
• Diagnostic Kit Manufacturing: Potential use as a component in enzymatic assays designed to measure glycosyltransferase activity or diagnose metabolic diseases.

Basic Information

Product Name	Mannosyl(5)-n-Acetyl(2)-Glucose Diphosphate Dolichol
CAS No.	119913-05-2
Molecular Formula	C95H153N2O26P2 (for Dolichol-19 chain length; varies)
Molecular Weight	~1800-2000 g/mol (Dependent on Dolichol chain length)
Synonyms	Dolichyl Diphosphate N-Acetylglucosaminyl Mannose; Dolichyl-PP-GlcNAc2Man5; GlcNAc2-Man5-PP-Dolichol; Lipid-Linked Oligosaccharide (LLO) precursor; N-Acetylglucosaminyldiphosphodolichol N-Acetylglucosaminyltransferase Substrate; Dol-PP-(GlcNAc)2(Man)5; Chitobiosyl Diphosphodolichol Mannose Derivative
EINECS	Contact for details

Quality Control

Our Mannosyl(5)-n-Acetyl(2)-Glucose Diphosphate Dolichol is produced and handled under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and biological suitability. We adhere to rigorous analytical protocols including HPLC, MS, and NMR to confirm structure and purity, meeting the exacting standards required for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store lyophilized material under argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white lyophilized solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Area %)
Assay (Enzymatic/Phosphate)	≥ 90%
Water Content (KF)	≤ 2.0%
Biological Activity	Functional as substrate for OST enzyme (Data provided)
Solubility	Soluble in chloroform/methanol/water mixtures

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.