Description

4-Nitrophenyl n-Trimethylammonioethyl Carbonate is a specialized quaternary ammonium salt derivative of a carbonate ester, primarily utilized as a highly reactive and chromogenic substrate for enzymatic assays. Its core value lies in its ability to serve as a key intermediate and diagnostic reagent in biochemical research and pharmaceutical development. This compound is essential for researchers and manufacturers in the fields of diagnostic kit production, enzyme kinetics studies, and proteomics, where precise and sensitive detection methods are required.

Application

• Enzymatic Substrate for Cholinesterases: Widely used as a chromogenic substrate for the spectrophotometric assay of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activity, crucial in neurological and toxicological research.
• Diagnostic Reagent Manufacturing: Serves as a key component in the formulation of clinical diagnostic kits for liver function, pesticide exposure, and nerve agent detection.
• Biochemical Research: Employed in kinetic studies and inhibitor screening for esterase enzymes, aiding in drug discovery for Alzheimer's disease and myasthenia gravis.
• Protease Activity Assays: Can be adapted for use in assays detecting specific protease activities through customized conjugate designs.
• Pharmaceutical Intermediate: Acts as a synthetic building block for more complex molecules targeting the cholinergic system.
• Quality Control in Agrochemicals: Used to test for cholinesterase-inhibiting activity of organophosphate and carbamate pesticides.

Basic Information

Product Name	4-Nitrophenyl n-Trimethylammonioethyl Carbonate
CAS No.	104407-34-3
Molecular Formula	C₁₄H₂₀N₂O₅⁺ (typically as an iodide or other salt)
Molecular Weight	Contact for details
Synonyms	4-Nitrophenyl (2-Trimethylammonioethyl) Carbonate; (2-Trimethylammonio)ethyl 4-Nitrophenyl Carbonate; 4-Nitrophenyl N-Trimethylammonioethyl Carbonate Iodide; Acetylthiocholine Analog Substrate; Cholinesterase Substrate; ATC; ChE Substrate
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl n-Trimethylammonioethyl Carbonate is produced under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive analytical applications. Each lot undergoes rigorous quality testing, including HPLC purity analysis, identity confirmation (IR/NMR), and functional activity validation in standardized enzyme assays. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent decomposition. For long-term stability, consider storage under desiccant.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Functional Activity (Enzymatic Assay)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.