Description

4-Nitrophenyl2-Acetamido-2-Deoxy-4,6-o-Benzylidene-B-D-Glucopyranoside is a specialized glycoside derivative, primarily utilized as a chromogenic substrate in enzymatic assays. This compound matters for its critical role in the study and quantification of glycosidase enzymes, providing a reliable and measurable colorimetric response upon enzymatic cleavage. It is essential for researchers and manufacturers in the fields of biochemistry, pharmaceutical development, and diagnostic reagent production who require high-purity, well-characterized substrates for accurate enzymatic analysis.

Application

• Enzymatic Substrate for Glycosidases: Acts as a specific chromogenic substrate for enzymes like β-N-acetylglucosaminidase and related glycosyl hydrolases.
• Biochemical Research: Used in kinetic studies, enzyme characterization, and inhibition assays to understand enzyme mechanisms and activity.
• Diagnostic Reagent Manufacturing: Incorporated into kits and reagents for the detection and measurement of specific enzyme activities in clinical and research laboratories.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) campaigns to identify potential therapeutic inhibitors of target glycosidases.
• Carbohydrate Chemistry Studies: Serves as a key intermediate or model compound in synthetic and analytical studies of complex carbohydrate structures and their interactions.

Basic Information

Item	Details
Product Name	4-Nitrophenyl2-Acetamido-2-Deoxy-4,6-o-Benzylidene-B-D-Glucopyranoside
CAS No.	19234-58-3
Molecular Formula	C21H22N2O8
Molecular Weight	430.41 g/mol
Synonyms	p-Nitrophenyl N-acetyl-β-D-glucosaminide, 4,6-O-benzylidene derivative; 4-Nitrophenyl 2-acetamido-2-deoxy-4,6-O-benzylidene-β-D-glucopyranoside; PNP-β-GlcNAc (benzylidene protected); NAG-4,6-O-benzylidene-β-pNP; 4-Nitrophenyl N-acetyl-4,6-O-benzylidene-β-D-glucosaminide; β-4-Nitrophenyl 2-acetamido-2-deoxy-4,6-O-benzylidene-D-glucopyranoside
EINECS	Contact for details

Quality Control

Every batch of 4-Nitrophenyl2-Acetamido-2-Deoxy-4,6-o-Benzylidene-B-D-Glucopyranoside is subjected to rigorous analytical testing to ensure identity, purity, and performance consistency suitable for sensitive biochemical applications. Our quality assurance protocols are designed to meet the exacting standards of research and diagnostic industries. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request to support your quality documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). This product is light-sensitive (store away from light) and should be handled to minimize exposure to moisture and air to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Specification available upon request
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.