Description

2-Nitrophenyl2-Acetamido-3,4,6-Tri-o-Acetyl-2-Deoxy-A-D-Glucopyranoside is a sophisticated, high-purity glycosylation intermediate and chromogenic substrate critical for advanced biochemical research and synthesis. Its value lies in its role as a key building block for the synthesis of complex oligosaccharides and glycoconjugates, enabling precise structural studies and the development of novel bioactive compounds. This compound is essential for researchers and manufacturers in pharmaceutical development, carbohydrate chemistry, and enzymology, particularly for studying glycosidase and glycosyltransferase activities.

Application

• Glycobiology Research: Serves as a chromogenic and fluorogenic substrate for the assay and characterization of β-N-acetylglucosaminidases and related enzymes.
• Oligosaccharide Synthesis: Acts as a key glycosyl donor or acceptor intermediate in the chemical and enzymatic synthesis of complex carbohydrate structures.
• Pharmaceutical Intermediate: Used in the research and development stages of novel glycotherapeutic agents, including antibiotics and antiviral drugs.
• Diagnostic Reagent Development: Employed in the formulation of enzyme-linked assays for clinical diagnostics and biochemical pathway analysis.
• Bioconjugation: Utilized for the site-specific labeling and modification of proteins and other biomolecules via its reactive glycosidic and acetyl groups.
• Academic & Industrial R&D: A fundamental tool for investigating glycosylation mechanisms, enzyme kinetics, and carbohydrate-protein interactions.

Basic Information

Item	Details
Product Name	2-Nitrophenyl2-Acetamido-3,4,6-Tri-o-Acetyl-2-Deoxy-A-D-Glucopyranoside
CAS No.	13264-91-0
Molecular Formula	C₂₁H₂₆N₂O₁₁
Molecular Weight	482.44 g/mol
Synonyms	p-Nitrophenyl N-acetyl-β-D-glucosaminide, tetraacetate; 4-Nitrophenyl 2-acetamido-2-deoxy-3,4,6-tri-O-acetyl-β-D-glucopyranoside; PNP-β-GlcNAc tetraacetate; 2-Acetamido-3,4,6-tri-O-acetyl-2-deoxy-β-D-glucopyranosyl 4-nitrophenyl ether; 4-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-β-D-glucopyranoside; β-4-Nitrophenyl N-acetylglucosaminide tetraacetate; NAG-4NP tetraacetate
EINECS	Contact for details

Quality Control

Every batch of 2-Nitrophenyl2-Acetamido-3,4,6-Tri-o-Acetyl-2-Deoxy-A-D-Glucopyranoside is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure compliance with high-purity standards suitable for research and development applications. A detailed Certificate of Analysis (COA) confirming identity, purity, and impurity profiles is provided with each shipment to guarantee batch-to-batch consistency and reliability for your critical work.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is moisture-sensitive (hygroscopic); ensure the container is kept in a dry environment to maintain stability and purity. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.