Description

Phenyl-β-D-Glucopyranoside is a specialized glycoside compound widely used as a chromogenic substrate in enzymatic assays. Its core value lies in its specificity for β-glucosidase enzymes, enabling precise biochemical research and diagnostic applications. This high-purity biochemical is essential for researchers and manufacturers in the pharmaceutical, diagnostic, and life science industries who require reliable substrates for enzyme activity studies and diagnostic kit development.

Application

• Enzymatic Substrate for β-Glucosidase: Primary use as a chromogenic substrate in spectrophotometric assays to measure β-glucosidase activity.
• Biochemical Research: Critical reagent for studying enzyme kinetics, inhibition, and the mechanism of action of glycoside hydrolases.
• Diagnostic Kit Manufacturing: Key component in the production of clinical diagnostic kits for genetic disorders like Gaucher's disease, where β-glucosidase activity is measured.
• Pharmaceutical Development: Used in high-throughput screening (HTS) platforms to identify potential drug candidates that modulate β-glucosidase activity.
• Microbiology & Plant Biology: Employed in research to detect and quantify β-glucosidase activity in microorganisms and plant extracts.
• Quality Control in Enzyme Production: Serves as a standard substrate for the quality assurance and potency testing of commercial β-glucosidase enzyme preparations.

Basic Information

Product Name	Phenyl-β-D-Glucopyranoside
CAS No.	1464-44-4
Molecular Formula	C12H16O6
Molecular Weight	256.25 g/mol
Synonyms	Phenyl β-D-Glucopyranoside; Phenyl β-D-Glucoside; β-D-Glucopyranosyloxybenzene; 4-Nitrophenyl-β-D-glucopyranoside (related compound); Phenyl-β-glucoside; Ph-β-Glc; Phenyl-β-glucoside; PBDG
EINECS	215-971-1

Quality Control

Our Phenyl-β-D-Glucopyranoside is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards and can provide material for qualification under cGMP guidelines for pharmaceutical development upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a dry environment to prevent absorption of moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Melting Point	168°C - 172°C
Specific Optical Rotation	-65° to -70° (c=1 in H2O)
Heavy Metals	≤ 10 ppm
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.