Description

Subtilisins are a group of serine proteolytic enzymes, primarily derived from *Bacillus* species, known for their broad substrate specificity and high alkaline stability. This makes them exceptionally valuable as powerful, biodegradable catalysts for hydrolyzing proteinaceous materials. They are essential biocatalysts for industrial manufacturers seeking efficient, sustainable, and high-performance processing solutions. Key industries that rely on these enzymes include detergents, leather processing, food & beverage, and pharmaceuticals.

Application

• Detergent & Cleaning Formulations: As a key active ingredient in laundry and dishwashing detergents for superior stain removal of protein-based soils (e.g., blood, egg, grass).
• Leather Processing: Used in the dehairing and bating stages to selectively degrade non-collagenous proteins, resulting in softer, higher-quality leather.
• Food & Beverage Industry: Applied in protein hydrolysis for flavor enhancement, meat tenderization, and the production of protein hydrolysates and savory flavors.
• Pharmaceutical & Diagnostic Applications: Utilized in peptide synthesis, wound debridement agents, and as a tool enzyme in biochemical research and diagnostics.
• Textile & Silk Processing: Employed for degumming silk fibers and bio-polishing of textiles to improve softness and appearance.
• Waste Management: Facilitates the biodegradation of protein-rich organic waste in environmental and industrial treatment processes.
• Contact Lens Cleaning Solutions: Acts as a proteolytic cleaner to remove protein deposits from contact lenses.
• Animal Feed Additive: Enhances protein digestibility and nutrient availability in feed formulations.

Basic Information

Product Name	Subtilisins
CAS No.	1395-21-7
Molecular Formula	N/A (Protein/Enzyme)
Molecular Weight	~27,000 Da (Approx., varies by isoform)
Synonyms	Subtilisin; Subtilopeptidase; Bacillus Subtilisin; Alkaline Protease; Serine Protease (Bacillus); Subtilisin Carlsberg; Subtilisin BPN'; Subtilisin E; Alcalase®; Savinase®; Esperase®; Protease S; Nagarse; Bacterial Proteinase
EINECS	215-752-5

Quality Control

Our Subtilisins are produced and tested under stringent quality management systems to ensure batch-to-batch consistency and performance. Quality parameters including proteolytic activity (U/g or PU), purity, and microbiological limits are rigorously controlled. Certificates of Analysis (COA) detailing specific assay results are provided with each shipment. We can supply grades suitable for industrial, food (FCC), and technical applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at 2-8°C (refrigerated). Under these conditions, the product is stable for the period indicated on the label. The material is hygroscopic (moisture-sensitive); keep the container tightly sealed in a dry environment. For bulk quantities, avoid repeated temperature fluctuations.

Specification

Item	Specification
Appearance	Light tan to brown powder or liquid
Identification (IR)	Conforms
Protease Activity	Min. 100,000 U/g (Grade dependent)
pH (Solution)	5.0 - 8.0
Loss on Drying	≤ 8.0 %
Total Viable Count	≤ 10,000 CFU/g
E. coli	Negative
Salmonella	Negative

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.