Description

Hydrogen [4-[[4-(diethylamino)phenyl][4-[ethyl[(3-sulphonatobenzyl)amino]-o-tolyl]methylene]-3-methylcyclohexa-2,5-dien-1-ylidene](ethyl)(3-sulphonatobenzyl)ammonium, sodium salt is a high-purity, water-soluble cyanine dye derivative. This compound is valued for its strong and tunable absorption and fluorescence properties in the visible to near-infrared spectrum. It is primarily sought after by research institutions and manufacturers in the biotechnology, diagnostics, and advanced materials sectors for its role as a sophisticated labeling and sensing agent.

Application

• Fluorescent Labeling Agent: Used for covalent or non-covalent tagging of biomolecules (proteins, nucleic acids) in bioassays and imaging.
• Biosensor Development: Serves as a key signaling component in optical sensors for detecting specific analytes.
• Photodynamic Therapy (PDT) Research: Investigated as a potential photosensitizer for targeted cancer treatments.
• Non-Linear Optical Materials: Incorporated into polymers and thin films for photonic and optoelectronic device research.
• Histological Staining: Employed in advanced microscopy techniques for cellular and tissue imaging.
• DNA/Protein Detection Kits: A critical raw material in the formulation of diagnostic kits and reagents.

Basic Information

Product Name	Hydrogen [4-[[4-(diethylamino)phenyl][4-[ethyl[(3-sulphonatobenzyl)amino]-o-tolyl]methylene]-3-methylcyclohexa-2,5-dien-1-ylidene](ethyl)(3-sulphonatobenzyl)ammonium, sodium salt
CAS No.	6505-30-2
Molecular Formula	C46H51N3O6S2•Na
Molecular Weight	~829.0 g/mol
Synonyms	Cy5 NHS ester analogue (sulphonated); Sulfo-Cyanine5 amine derivative; Heptamethine cyanine dye, sulfonated benzyl amino substituted; 6505-30-2; Sodium 4-[[4-(diethylamino)phenyl][4-[ethyl[(3-sulfonatobenzyl)amino]-2-methylphenyl]methylene]-3-methyl-1-cyclohexa-2,5-dienylidene]ethyl(3-sulfonatobenzyl)ammonium; Water-soluble Cy5 dye; Fluorescent probe 6505-30-2.
EINECS	Contact for details

Quality Control

Our production of this advanced dye adheres to strict quality management protocols. Each batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure structural identity and purity. Certificates of Analysis (COA) detailing lot-specific purity, absorbance/fluorescence profiles, and impurity limits are provided to guarantee consistency for your critical research and manufacturing applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term stability, consider storage under inert atmosphere.

Specification

Item	Specification
Appearance	Dark green to blue-green solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0% (Area %)
Water Content (KF)	≤ 5.0 %
Absorption Max (λmax)	646 - 652 nm (in aqueous buffer)
Extinction Coefficient (ε)	≥ 200,000 L·mol⁻¹·cm⁻¹
Single Largest Unknown Impurity (HPLC)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.