Description

Ac-Ala-Ala-Tyr-Amc CAS NO 99590-93-9 is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control labs requiring precise measurement of protease activity, particularly chymotrypsin and related enzymes. It is primarily utilized in pharmaceutical R&D, biochemical research, and diagnostic kit development where accurate, real-time kinetic analysis is essential.

Application

• Protease Activity Assays: As a selective fluorogenic substrate for monitoring chymotrypsin, subtilisin, and other serine proteases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel protease inhibitors.
• Biochemical Research: A key tool for studying enzyme kinetics, mechanism of action, and substrate specificity in academic and industrial laboratories.
• Diagnostic Development: Employed in the formulation of sensitive diagnostic tests and kits for clinical and research purposes.
• Quality Control: Applied in biopharmaceutical manufacturing to monitor and validate the activity and purity of enzyme-based products.

Basic Information

Product Name	Ac-Ala-Ala-Tyr-Amc
CAS No.	99590-93-9
Molecular Formula	C₂₆H₃₀N₄O₇
Molecular Weight	510.54 g/mol
Synonyms	N-Acetyl-Ala-Ala-Tyr-7-amido-4-methylcoumarin; Acetyl-Alanyl-Alanyl-Tyrosyl-AMC; Ac-AAY-AMC; Ac-AAY-AMC Fluorogenic Substrate; Chymotrypsin Substrate I; Suc-AAY-AMC (common alternate); N-Acetyl-L-alanyl-L-alanyl-L-tyrosine 7-amido-4-methylcoumarin
EINECS	Contact for details

Quality Control

Our Ac-Ala-Ala-Tyr-Amc is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data. We adhere to relevant industry standards for research-grade biochemicals, ensuring reliability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or moisture will compromise stability and performance. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF
Fluorogenic Activity	Functional test provided (COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.