Description

Sulforhodamine B 2-Acid Fluoride is a highly reactive, activated derivative of the classic fluorescent dye Sulforhodamine B, designed for efficient covalent labeling. This compound is crucial for researchers and manufacturers requiring stable, site-specific conjugation of fluorescent probes to biomolecules, surfaces, or polymers. It is primarily needed by professionals in bioconjugation chemistry, diagnostic assay development, and advanced materials science.

Application

• Fluorescent Labeling Reagent: For the covalent attachment of a bright, red-emitting fluorophore to antibodies, proteins, peptides, and oligonucleotides.
• Diagnostic Kit Manufacturing: Used in the production of fluorescent immunoassays (FLISA), lateral flow tests, and flow cytometry reagents.
• Biomaterial and Polymer Modification: Functionalizing nanoparticles, hydrogels, and polymer surfaces with fluorescent tags for tracking and imaging.
• Cell and Tissue Imaging Probes: Synthesis of targeted fluorescent dyes for microscopy applications in biological research.
• Chemical Biology Tools: Creating activity-based probes or affinity labels for studying enzyme function or protein interactions.
• Quality Control & Standardization: Acting as a reference standard or a key intermediate in the quality control of fluorescent diagnostic products.

Basic Information

Product Name	Sulforhodamine B 2-Acid Fluoride
CAS No.	98806-82-7
Molecular Formula	C27H20F3N2O7S2
Molecular Weight	605.58 g/mol
Synonyms	Sulforhodamine B Acid Fluoride; Texas Red-X Acid Fluoride; Texas Red-X Succinimidyl Ester analogue (acid fluoride form); 5-(and-6)-Carboxy-X-rhodamine Acid Fluoride; SRB 2-AF; Lissamine Rhodamine B Acid Fluoride; Sulforhodamine 101 Acid Fluoride derivative; Reactive red fluorescent dye acid fluoride
EINECS	Contact for details

Quality Control

Our Sulforhodamine B 2-Acid Fluoride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive bioconjugation work. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence properties, and moisture content is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This material is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Area %)
Absorption Maximum (λmax)	580 - 590 nm
Emission Maximum	600 - 610 nm
Moisture Content (KF)	≤ 1.0 %
Functional Group Assay	≥ 90% (Acid Fluoride content)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.