Description

Histopaque-1077 is a sterile, ready-to-use density gradient medium specifically formulated for the isolation of viable mononuclear cells from human peripheral blood and bone marrow. This product is critical for ensuring high cell yield and purity in downstream research and diagnostic applications. It is an essential reagent for laboratories in the fields of immunology, cell therapy, cancer research, and clinical diagnostics, where reliable and consistent cell separation is paramount.

Application

• Peripheral Blood Mononuclear Cell (PBMC) Isolation: Primary application for separating lymphocytes and monocytes from whole blood for immunological studies.
• Bone Marrow Processing: Isolation of mononuclear cells from bone marrow aspirates for hematopoietic stem cell research and transplantation.
• Cell Therapy Manufacturing: A key initial step in the production of cell-based therapies, including CAR-T and other adoptive immunotherapies.
• Virology and Infectious Disease Research: Preparation of target host cells for in vitro infection studies and viral propagation.
• Cancer Research: Isolation of tumor-infiltrating lymphocytes (TILs) or circulating tumor cells (CTCs) from patient samples.
• Vaccine Development: Used in the preparation of antigen-presenting cells and other immune cells for vaccine efficacy testing.
• General Cell Culture: Provides a purified population of mononuclear cells for establishing primary cultures.
• Diagnostic Testing: Sample preparation for flow cytometry, molecular diagnostics, and other clinical lab tests.

Basic Information

Product Name	Histopaque-1077
CAS No.	97639-11-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Ficoll-Paque; Polysucrose 400; Sodium diatrizoate; Lymphocyte Separation Medium (LSM); Density Gradient Medium; Ficoll-Hypaque; Cell Separation Medium; Ficoll 400 – Sodium Diatrizoate Solution
EINECS	Contact for details

Quality Control

Histopaque-1077 is manufactured under strict quality systems. Each batch is tested for sterility (by membrane filtration), endotoxin levels (LAL test), osmolality, density, pH, and functionality in cell separation to ensure consistent performance. A Certificate of Analysis (COA) is provided with each shipment, detailing these specifications. Our quality commitment supports applications requiring GMP-like materials for clinical and advanced research use.

Storage

Preserve in the original, unopened bottle in a tightly closed container, protected from light. Store at 15-25°C. Do not freeze. Once opened, use promptly under aseptic conditions to maintain sterility. Keep the container tightly sealed when not in use to prevent evaporation or microbial contamination.

Specification

Item	Specification
Appearance	Clear, colorless to pale yellow solution
Density (at 20°C)	1.077 g/mL ± 0.001 g/mL
Osmolality	290 ± 15 mOsm/kg
pH	7.2 ± 0.2
Sterility	Sterile (Membrane Filtration)
Endotoxin	< 0.12 EU/mL (LAL test)
Functionality (Cell Recovery)	> 90% viable mononuclear cells
Functionality (Cell Viability)	> 95% by Trypan Blue exclusion

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.