Description

n-Ethyl-n-(2-Hydroxy-3-Sulfopropyl)-3,5-Dimethylaniline Sodium Salt Monohydrate is a specialized organic salt widely recognized as a key chromogenic reagent in enzymatic diagnostic assays. Its primary value lies in its ability to produce a distinct, measurable color change when acted upon by specific enzymes like peroxidase, enabling precise quantitative analysis. This makes it an essential component for manufacturers of in-vitro diagnostic (IVD) kits and clinical chemistry reagents, particularly for tests measuring glucose, cholesterol, and uric acid.

Application

• Primary Chromogen in Clinical Diagnostics: Used as a Trinder's reagent in enzymatic colorimetric assays for the quantitative determination of metabolites in blood serum and plasma.
• Glucose Test Kits: A critical reagent in glucose oxidase/peroxidase (GOD-POD) based systems for blood glucose monitoring.
• Cholesterol & Triglyceride Assays: Employed in enzymatic methods for lipid profile testing in clinical laboratories.
• Uric Acid Determination: Key component in uricase-based diagnostic kits.
• Research & Development: Serves as a model substrate in biochemical research for studying peroxidase enzyme kinetics and developing new diagnostic methodologies.
• Veterinary Diagnostics: Adapted for use in clinical chemistry analyzers for animal health testing.
• Quality Control Reagents: Used in the production of control sera and calibrators for diagnostic equipment.

Basic Information

Product Name	n-Ethyl-n-(2-Hydroxy-3-Sulfopropyl)-3,5-Dimethylaniline Sodium Salt Monohydrate
CAS No.	82692-97-5
Molecular Formula	C14H22NNaO5S • H2O
Molecular Weight	351.39 g/mol (Monohydrate)
Synonyms	DAOS; N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt; 3,5-Dimethoxy-N-ethyl-N-(2-hydroxy-3-sulfopropyl)aniline sodium salt; DAOS Sodium Salt; Chromogen DAOS; 4-Amino-2,6-dimethoxyphenol derivative; Trinder's Reagent DAOS; Diagnostic reagent DAOS; EHSDAS Sodium Salt
EINECS	Contact for details

Quality Control

Our production of n-Ethyl-n-(2-Hydroxy-3-Sulfopropyl)-3,5-Dimethylaniline Sodium Salt Monohydrate adheres to stringent quality management systems suitable for diagnostic reagent manufacture. Every batch undergoes comprehensive analytical testing to ensure high purity, consistent performance, and low background absorbance, which are critical for reliable diagnostic results. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive); therefore, the container must be kept tightly sealed in a dry environment to prevent absorption of moisture, which can affect performance and stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 98.0%
Water Content (KF)	4.5% - 6.0% (Theoretical for monohydrate: ~5.1%)
Absorbance (Specific Test)	Meets performance criteria in model assay
Heavy Metals (as Pb)	≤ 20 ppm
Solubility (in water)	Clear, colorless solution

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.