Description

Sma I CAS NO 82391-42-2 is a high-purity restriction enzyme, a critical tool for precise DNA manipulation in molecular biology research and biotechnological applications. This endonuclease is valued for its specific recognition and cleavage of DNA sequences, enabling accurate genetic analysis and recombinant DNA technology. It is an essential reagent for pharmaceutical R&D, academic research institutions, and diagnostic kit manufacturers requiring reliable and consistent enzymatic activity for genetic engineering workflows.

Application

• Molecular Cloning: Essential for the precise cutting and insertion of DNA fragments into vectors during plasmid construction.
• Genetic Engineering: Used in the development of genetically modified organisms (GMOs) and gene therapy constructs.
• Restriction Fragment Length Polymorphism (RFLP) Analysis: A key enzyme for DNA fingerprinting, mutation detection, and genetic mapping.
• Diagnostic Assay Development: Employed in the creation of molecular diagnostic tests for pathogen detection and genetic disorder screening.
• Genome Sequencing Projects: Facilitates the physical mapping and assembly of genomic DNA.
• Biopharmaceutical Production: Used in the R&D and quality control of biologics, including monoclonal antibodies and recombinant proteins.

Basic Information

Product Name	Sma I
CAS No.	82391-42-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	SmaI; Endodeoxyribonuclease SmaI; Restriction enzyme Sma I; Serratia marcescens Sma I; CCCGGG-specific type II restriction enzyme; EC 3.1.21.4
EINECS	Contact for details

Quality Control

Our Sma I is produced under stringent quality management systems to ensure batch-to-batch consistency and optimal enzymatic activity. Each lot is tested for specific activity, purity (free from non-specific nucleases and phosphatases), and stability. A comprehensive Certificate of Analysis (COA) detailing purity, activity units, and storage buffer composition is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C. The product is hygroscopic (moisture-sensitive). Avoid repeated freeze-thaw cycles. Aliquot into working volumes upon receipt to maintain optimal activity.

Specification

Item	Specification
Appearance	Clear colorless solution
Identification (Activity)	Cleaves CCCGGG sequence specifically
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Non-Specific Nuclease Activity	Not detected
Phosphatase Activity	Not detected
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.