Description

Pvu Ii CAS NO 81295-34-3 is a high-purity restriction enzyme, a critical tool for molecular biology research and biotechnological applications. This enzyme is essential for precise DNA manipulation, enabling accurate genetic analysis, cloning, and sequencing workflows. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers requiring reliable and specific DNA cleavage.

Application

• Molecular Cloning: Used for the precise insertion of DNA fragments into vectors.
• Genetic Engineering: Facilitates gene knockout, mutagenesis, and the construction of recombinant DNA molecules.
• DNA Mapping & Analysis: Essential for restriction fragment length polymorphism (RFLP) analysis and genomic mapping.
• Diagnostic Assay Development: Employed in the design of tests for genetic disorders and pathogen detection.
• Next-Generation Sequencing (NGS) Library Preparation: Used in workflows for fragmenting and preparing DNA samples for sequencing.
• Biopharmaceutical Production: Applied in the development and quality control of plasmid DNA used in gene therapies and vaccines.

Basic Information

Product Name	Pvu Ii
CAS No.	81295-34-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	PvuII Restriction Endonuclease; Endodeoxyribonuclease PvuII; Restriction Enzyme PvuII; PvuII (Providencia stuartii); EC 3.1.21.4; Type II Restriction Enzyme PvuII
EINECS	Contact for details

Quality Control

Our Pvu Ii is manufactured under stringent quality systems. Each batch undergoes rigorous testing for activity, specificity, and the absence of contaminating nucleases to ensure performance consistency. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity, and storage buffer composition is provided with every shipment to guarantee it meets the high standards required for sensitive molecular biology applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C. This product is hygroscopic (moisture-sensitive). For long-term stability, avoid repeated freeze-thaw cycles and aliquot into single-use volumes upon receipt.

Specification

Item	Specification
Appearance	Clear colorless solution
Identification (Activity)	Cleaves 1 µg of λ DNA completely in 1 hour under recommended conditions
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Contaminating Nuclease Activity	Not detected
Storage Buffer	10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 µg/ml BSA, 50% Glycerol, pH 7.4 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.