Description

Methyl 3-Imidate-2,2,5,5-Tetramethylpyrroline 1-Oxide is a specialized nitroxide spin label derivative, a crucial intermediate in the synthesis of advanced spin probes for Electron Paramagnetic Resonance (EPR) spectroscopy. This compound matters because it enables the site-directed spin labeling of biomolecules, allowing researchers to study protein dynamics, conformational changes, and molecular interactions with high precision. It is primarily needed by research institutions, pharmaceutical R&D departments, and chemical manufacturers developing novel diagnostic tools and studying complex biochemical systems.

Application

• Spin Labeling Reagent: Key intermediate for synthesizing methanethiosulfonate (MTSSL) and other thiol-reactive spin labels for site-directed labeling of cysteine residues in proteins and peptides.
• EPR Spectroscopy: Used in the preparation of nitroxide spin probes for studying protein structure, dynamics, folding, and ligand binding via continuous wave and pulsed EPR techniques.
• Biophysical Research: Enables the investigation of membrane protein topology, lipid bilayer properties, and nucleic acid structure through spin labeling methodologies.
• Structural Biology: Aids in determining distances and orientations within biomolecular complexes using techniques like Double Electron-Electron Resonance (DEER or PELDOR).
• Pharmaceutical Development: Utilized in drug discovery to monitor conformational changes in target proteins upon inhibitor or drug candidate binding.
• Material Science: Can be incorporated into polymers or surfaces to create spin-labeled materials for studying interfacial phenomena and material properties.

Basic Information

Product Name	Methyl 3-Imidate-2,2,5,5-Tetramethylpyrroline 1-Oxide
CAS No.	77044-72-5
Molecular Formula	C10H17N2O2
Molecular Weight	197.26 g/mol
Synonyms	3-Imidate-2,2,5,5-Tetramethylpyrroline-1-oxyl Methyl Ester; Methyl 3-Imidate-2,2,5,5-Tetramethyl-1-pyrrolinyloxy; Methyl 3-Imidate-TEMPO; 2,2,5,5-Tetramethyl-3-imidate-pyrrolidine-1-oxyl Methyl Ester; MTSSL Precursor; Nitroxide Spin Label Imidate; TEMPO Methyl Imidate; 1-Oxyl-2,2,5,5-tetramethyl-δ3-pyrroline-3-methyl imidate
EINECS	Contact for details

Quality Control

Our Methyl 3-Imidate-2,2,5,5-Tetramethylpyrroline 1-Oxide is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity, critical for sensitive spectroscopic applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity assessment and EPR to confirm the integrity of the nitroxide radical. Certificates of Analysis (COA) detailing identity, purity, and specific tests are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). For long-term stability, consider storage under an inert atmosphere such as argon or nitrogen, especially after the container has been opened, to minimize potential degradation. Keep away from strong reducing agents.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder or solid
Identification (IR)	Conforms to structure
Identification (EPR)	Characteristic nitroxide radical signal present
Purity (HPLC)	≥ 95% (Area %)
Melting Point	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.