Description

n-Succinimidyl S-Acetylthioglycolate is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a protected thiol group. This compound is critically important for the site-specific conjugation of biomolecules, enabling the creation of stable, well-defined protein-drug conjugates and antibody-enzyme complexes. It is an essential tool for researchers and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay production, where controlled linkage and functional integrity are paramount.

Application

• Antibody-Drug Conjugate (ADC) Linker Chemistry: Serves as a key intermediate for constructing cleavable or non-cleavable linkers that connect cytotoxic payloads to monoclonal antibodies.
• Protein and Peptide Labeling: Used to introduce protected thiol groups onto amines, which can later be deprotected for site-specific attachment of fluorescent dyes, biotin, or other probes.
• Surface Immobilization: Facilitates the oriented covalent immobilization of proteins, antibodies, or enzymes onto solid supports (e.g., biosensor chips, microplates, beads) via amine coupling with subsequent thiol-based capture.
• Polymer and Biomaterial Functionalization: Enables the modification of amine-containing polymers or hydrogels with thiol-reactive sites for further crosslinking or ligand attachment.
• Diagnostic Reagent Development: Employed in the synthesis of enzyme-antibody conjugates and other detection complexes used in ELISA, lateral flow assays, and other immunoassays.
• Nanoparticle Conjugation: Used to functionalize the surface of nanoparticles (e.g., gold, quantum dots) with biomolecules for targeted delivery or imaging applications.

Basic Information

Product Name	n-Succinimidyl S-Acetylthioglycolate
CAS No.	76931-93-6
Molecular Formula	C8H9NO5S
Molecular Weight	231.22 g/mol
Synonyms	S-Acetylthioglycolic Acid N-Succinimidyl Ester; SATP; NHS-SATA; N-Succinimidyl S-Acetylthioacetate; SATA; S-Acetylmercaptosuccinic Acid N-Hydroxysuccinimide Ester; 2,5-Dioxopyrrolidin-1-yl 2-(acetylthio)acetate; N-(2,5-Dioxopyrrolidin-1-yl)-2-(acetylthio)acetamide
EINECS	Contact for details

Quality Control

Our n-Succinimidyl S-Acetylthioglycolate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive bioconjugation applications. Each lot undergoes comprehensive analytical testing including HPLC for purity and identity confirmation (NMR, IR). Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and the active NHS ester is susceptible to hydrolysis. For long-term stability, store in a freezer. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.