Description

Dimethyl Malonoimidate Dihydrochloride is a versatile bifunctional imidoester crosslinking agent, primarily used for protein modification and peptide synthesis. Its high reactivity and specificity make it a critical reagent for creating stable conjugates and modifying biomolecules under mild conditions. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors, where precise molecular conjugation is required for drug development, antibody labeling, and assay development.

Application

• Protein Crosslinking and Conjugation: Used to create stable amide bonds between proteins, antibodies, and other biomolecules for therapeutic and diagnostic applications.
• Peptide Synthesis Intermediate: Serves as a key building block in solid-phase and solution-phase peptide synthesis.
• Bioconjugate Chemistry: Facilitates the attachment of drugs, labels (e.g., fluorescent dyes), or polymers to proteins and peptides.
• Immunoassay Development: Employed in the preparation of enzyme-antibody conjugates for ELISA and other immunoassay platforms.
• Affinity Chromatography Ligand Immobilization: Used to covalently attach ligands to chromatographic supports for protein purification.
• Material Surface Functionalization: Applied to modify surfaces with reactive amine groups for biosensor and biomaterial development.
• Research & Development: A valuable tool in academic and industrial R&D for studying protein-protein interactions and structure-function relationships.

Basic Information

Product Name	Dimethyl Malonoimidate Dihydrochloride
CAS No.	71160-05-9
Molecular Formula	C5H10N2O2 • 2HCl
Molecular Weight	215.07 g/mol
Synonyms	Dimethyl Malonoimidate Dihydrochloride; Malonoimidic Acid, Dimethyl Ester, Dihydrochloride; Dimethyl Malonimidate Dihydrochloride; DMMI•2HCl; NSC 102601; Methyl 3-(Methoxycarbonimidoyl)propanoate Dihydrochloride; Dimethyl Malonimidate Hydrochloride
EINECS	Contact for details

Quality Control

Our Dimethyl Malonoimidate Dihydrochloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and titration for assay determination, to ensure it meets the high standards required for research and bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurities is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay (Titration)	95.0 - 105.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.