Description

Nile Red CAS NO 7385-67-3 is a highly versatile and intensely fluorescent benzophenoxazine dye. It is a critical tool for the selective staining and quantification of intracellular lipid droplets and hydrophobic domains within biological and material science research. This compound is essential for researchers and quality control professionals requiring precise, sensitive detection of lipophilic substances. Its primary applications span across biotechnology, pharmaceutical development, and advanced materials characterization.

Application

• Lipid Droplet Staining & Quantification: A vital fluorescent probe for visualizing and analyzing neutral lipid content in live or fixed cells, crucial for metabolic disorder and obesity research.
• Polymer & Material Science: Used to study polymer morphology, monitor curing processes, and detect hydrophobic microdomains in block copolymers and composite materials.
• Environmental Monitoring: Employed in the detection and analysis of hydrophobic organic contaminants and petroleum hydrocarbons in environmental samples.
• Microbiology & Biofilm Studies: Applied to stain and evaluate the lipid composition of microbial cells and the extracellular polymeric matrix of biofilms.
• Cosmetic & Dermatological Research: Utilized in formulations and skin models to assess sebum production, lipid barrier function, and product penetration.
• Food Science & Quality Control: Assists in the analysis of fat content, distribution, and oxidation in food products.
• Diagnostic Assay Development: Serves as a key component in developing fluorescence-based assays for clinical diagnostics and high-throughput screening.

Basic Information

Item	Details
Product Name	Nile Red
CAS No.	7385-67-3
Molecular Formula	C20H18N2O2
Molecular Weight	318.37 g/mol
Synonyms	Nile Blue A Oxazone; 9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one; Solvent Red 149; Nile Red Fluorescent Dye; LipidTOX™ Red (analog); 9-Diethylamino-5H-benzo[alpha]phenoxazine-5-one
EINECS	Contact for details

Quality Control

Our Nile Red is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic analysis for identity confirmation. A Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent photodegradation.

Specification

Item	Specification
Appearance	Dark red to brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0 %
Fluorescence Properties	Ex/Em maxima consistent with reference
Solubility	Soluble in DMSO, ethanol, acetone; insoluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.