Description

Thionine is a synthetic organic dye belonging to the phenothiazine class, recognized for its distinctive deep violet color and redox-active properties. This compound is a vital reagent in analytical chemistry and life sciences, serving as a sensitive redox indicator and biological stain. It is essential for research and industrial applications in histology, microbiology, and electrochemical sensor development, where precise colorimetric and electrochemical responses are required.

Application

• Biological Staining: Used as a metachromatic dye for staining nucleic acids (DNA/RNA) and acidic tissue components in histology and cytology.
• Microbiological Indicator: Employed as a redox indicator in culture media to differentiate bacterial species based on their metabolic activity.
• Electrochemical Analysis: Acts as a mediator in biosensors and electrochemical cells due to its reversible redox behavior.
• Photodynamic Therapy Research: Investigated for its potential as a photosensitizer in experimental photochemical and photobiological studies.
• Analytical Chemistry: Serves as a standard or reagent in spectrophotometric and colorimetric determination methods.
• Teaching & Research: A common laboratory chemical for educational demonstrations and fundamental research in chemistry and biology.

Basic Information

Product Name	Thionine
CAS No.	581-64-6
Molecular Formula	C12H10N3S+
Molecular Weight	228.29 g/mol (cation)
Synonyms	Lauth's Violet; Thionine Acetate; Thionine Chloride; Thionine Blue; 3,7-Diamino-5-phenothiazinium; CI 52000; Basic Blue 25; AI3-52000; Phenothiazin-5-ium, 3,7-diamino-
EINECS	209-480-2

Quality Control

Our Thionine is produced and tested under a strict quality management system to ensure batch-to-batch consistency and reliability. We provide material that meets high-purity standards suitable for research and analytical applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is supplied with each shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept tightly sealed after each use to prevent degradation and absorption of moisture.

Specification

Item	Specification
Appearance	Dark green to violet crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Absorbance (λ max)	599 - 603 nm (in water)
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.