Description

2-(4-Nitrophenyl)-4,4,5,5-Tetramethylimidazoline-3-Oxide-1-Oxyl is a stable nitroxide radical compound, widely recognized as a spin label and probe in advanced spectroscopic research. Its primary value lies in its paramagnetic properties, making it an essential tool for studying molecular dynamics, protein structure, and polymer systems through techniques like Electron Paramagnetic Resonance (EPR) spectroscopy. This high-purity chemical is critical for researchers and developers in the fields of biophysics, materials science, and pharmaceutical development who require reliable, well-characterized spin labels.

Application

• Spin Labeling in Biophysics: Used to tag proteins, nucleic acids, and lipids to study conformational changes, dynamics, and interactions via EPR spectroscopy.
• Polymer Science & Materials Research: Acts as a radical probe to investigate polymer chain mobility, cross-linking density, and degradation mechanisms.
• Antioxidant Activity Studies: Serves as a stable radical standard in assays measuring the radical scavenging capacity of novel compounds and natural extracts.
• Membrane & Lipid Bilayer Studies: Employed to probe membrane fluidity, phase transitions, and the effects of incorporated molecules on lipid order.
• Structural Biology: Utilized in site-directed spin labeling (SDSL) for determining protein structure and folding pathways.
• Catalysis & Reaction Mechanism Analysis: Functions as a paramagnetic tracer or inhibitor to elucidate reaction pathways in chemical and enzymatic catalysis.

Basic Information

Product Name	2-(4-Nitrophenyl)-4,4,5,5-Tetramethylimidazoline-3-Oxide-1-Oxyl
CAS No.	38582-73-9
Molecular Formula	C13H16N3O4
Molecular Weight	278.29 g/mol
Synonyms	4-Nitro-TEMPO; 4-Nitrophenyl-TEMPO; 2-(4-Nitrophenyl)-4,4,5,5-tetramethyl-4,5-dihydro-1H-imidazol-1-oxyl 3-oxide; 4-Nitro-2,2,6,6-tetramethylpiperidine-1-oxyl (common misnomer); 4-Nitrophenyl-substituted nitroxide; 4-NO2-TEMPO; TEMPO Nitrophenyl Derivative; Nitroxide Spin Label 4-Nitrophenyl
EINECS	Contact for details

Quality Control

Our 2-(4-Nitrophenyl)-4,4,5,5-Tetramethylimidazoline-3-Oxide-1-Oxyl is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, which is paramount for sensitive spectroscopic applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and EPR for radical concentration verification. Certificates of Analysis (COA) detailing all specifications are provided to guarantee the material meets the stringent requirements of research and development laboratories.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is light-sensitive (store away from light) and should be handled under an inert atmosphere when appropriate to minimize potential degradation. Keep away from strong reducing agents.

Specification

Item	Specification
Appearance	Orange to red crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
EPR Analysis	Confirms paramagnetic nitroxide radical
Melting Point	Contact for details
Residue on Ignition	< 0.5%
Solubility	Soluble in common organic solvents (e.g., DMSO, Chloroform, Ethanol)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.