Description

Naphthol As-D Chloroacetate is a specialized organic chemical intermediate, primarily recognized for its role as a chromogenic substrate in diagnostic histochemistry. Its core value proposition lies in its ability to produce a distinct, insoluble colored precipitate upon enzymatic hydrolysis, enabling precise localization of specific enzymes in tissue samples. This compound is essential for research and diagnostic laboratories in the fields of pathology, histology, and life sciences. Its reliable performance is critical for accurate staining and analysis in enzyme histochemistry applications.

Application

• Primary substrate for the detection of chloroacetate esterase (CAE) activity in enzyme histochemistry.
• Key reagent in the identification and classification of myeloid cell lineages, particularly granulocytes and mast cells, in bone marrow and blood smears.
• Used in diagnostic kits and protocols for hematological malignancies, aiding in the differentiation of acute leukemias (e.g., AML vs. ALL).
• Essential component in research histochemistry for studying enzyme distribution and activity in various biological tissues.
• Applied in frozen section and paraffin-embedded tissue staining procedures in clinical pathology laboratories.
• Supports quality control and method development for in-vitro diagnostic (IVD) manufacturers.

Basic Information

Product Name	Naphthol As-D Chloroacetate
CAS No.	35245-26-2
Molecular Formula	C19H16ClNO3
Molecular Weight	341.79 g/mol
Synonyms	Naphthol AS-D Chloroacetate; 3-Hydroxy-2-naphthoic acid 2-chloroanilide chloroacetate ester; 2-Chloro-N-(3-hydroxynaphthalen-2-yl)acetamide; Naphthol ASD Chloroacetate; AS-D Chloroacetate; Chloroacetate ester of Naphthol AS-D; Naphthol AS-D chloroacetic acid ester; 2-Naphthol, 3-hydroxy-, 2-chloroacetate
EINECS	Contact for details

Quality Control

Our Naphthol As-D Chloroacetate is manufactured under strict quality management systems to ensure batch-to-batch consistency and performance reliability for critical diagnostic applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR), to meet exacting standards. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substance profiles is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed immediately after use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Melting Point	158°C - 162°C
Loss on Drying	≤ 1.0%
Heavy Metals	≤ 20 ppm
Solubility (Test)	Freely soluble in DMF, sparingly soluble in ethanol
Performance (Staining Test)	Produces characteristic red granulation in control tissue sections

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.