Description

Dichlorofluorescein Sodium Salt is a high-purity, water-soluble derivative of fluorescein, widely recognized for its utility as a sensitive fluorescent probe and indicator. Its core value lies in its ability to act as a precursor for the detection of reactive oxygen species (ROS) and as a versatile staining agent in analytical biochemistry. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries, where precise detection and quantification are paramount.

Application

• ROS Detection Probe: Primarily used as a non-fluorescent precursor (DCFH) for the sensitive fluorometric detection and quantification of intracellular reactive oxygen species (ROS), such as hydrogen peroxide and peroxynitrite, in cellular assays.
• Biochemical Staining: Employed as a fluorescent stain for visualizing cellular components, proteins, and nucleic acids in gel electrophoresis and microscopy applications.
• Oxidation-Reduction Indicator: Serves as a redox-sensitive dye in various enzymatic and chemical reaction studies to monitor oxidation states and reaction kinetics.
• Diagnostic Reagent Manufacturing: Used in the formulation of diagnostic kits and reagents for clinical and research purposes, particularly in assays measuring oxidative stress.
• Pharmaceutical Research: Applied in drug discovery and development to screen for antioxidant activity and to study mechanisms of oxidative damage and cell apoptosis.
• Analytical Chemistry Standard: Functions as a reference standard and calibrant in high-performance liquid chromatography (HPLC) and other analytical methods.

Basic Information

Product Name	Dichlorofluorescein Sodium Salt
CAS No.	3474-67-7
Molecular Formula	C20H9Cl2NaO5
Molecular Weight	423.18 g/mol
Synonyms	2',7'-Dichlorofluorescein Sodium Salt; DCF Sodium Salt; Sodium 2',7'-Dichlorofluorescein; 3',6'-Dichlorofluoran Sodium Salt; DCFH Sodium Salt; Dichlorofluorescein, Sodium Derivative; Reactive Oxygen Species Indicator; Fluorescein, 2',7'-dichloro-, sodium salt
EINECS	Contact for details

Quality Control

Our Dichlorofluorescein Sodium Salt is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via IR and UV-Vis spectroscopy. A Certificate of Analysis (COA) detailing all test results and specifications is provided with every shipment to guarantee compliance with your application requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to air, moisture, or light may affect its stability and performance.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
UV-Vis Absorption	Conforms
Water Content (KF)	≤ 5.0%
Residue on Ignition	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.