Description

4-Nitrofluorescein is a chemically modified derivative of fluorescein, characterized by the introduction of a nitro group which significantly alters its spectroscopic and chemical properties. This compound is valued for its role as a key synthetic intermediate and a versatile precursor in the development of specialized dyes, fluorescent probes, and biological staining agents. It is primarily utilized by research institutions, diagnostic kit manufacturers, and fine chemical producers in the life sciences, analytical chemistry, and advanced materials sectors. The product is supplied under the identifier CAS NO 3326-35-0.

Application

• Fluorescent Probe Synthesis: Acts as a crucial precursor for creating advanced fluorescent tags and sensors used in bioimaging and cellular analysis.
• Biochemical Staining: Employed in the development of specialized stains for microscopy and histology to visualize cellular structures.
• Diagnostic Reagents: Used in the formulation of reagents for clinical diagnostics and research assay kits.
• Organic Synthesis Intermediate: Serves as a building block for synthesizing more complex xanthene dyes and photostable compounds.
• Photodynamic Therapy Research: Investigated as a potential photosensitizer precursor in medical research applications.
• Analytical Chemistry Standards: Utilized as a reference standard in chromatographic and spectroscopic method development.

Basic Information

Product Name	4-Nitrofluorescein
CAS No.	3326-35-0
Molecular Formula	C20H11NO7
Molecular Weight	377.30 g/mol
Synonyms	4-Nitrofluorescein; Fluorescein, 4-nitro-; 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-5-nitrobenzoic acid; 4-Nitrofluorane; 9-(2-Carboxy-4-nitrophenyl)-6-hydroxy-3H-xanthen-3-one; C.I. 45350:1; Nitrofluorescein
EINECS	222-045-2

Quality Control

Our 4-Nitrofluorescein is manufactured under a quality management system and undergoes rigorous analytical testing to ensure batch-to-batch consistency and high purity. Standard quality parameters include identity confirmation, purity assay, and control of key impurities. A Certificate of Analysis (COA) detailing specific results is provided with each shipment. We can support compliance requirements for research and industrial applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at a controlled room temperature (typically 15-25°C). Keep away from incompatible materials. Due to its light-sensitive nature, prolonged exposure to direct light should be avoided.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0 %
Residue on Ignition	≤ 0.5 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.