Description

m-Peg4-Dbco CAS NO 2228857-36-9 is a heterobifunctional crosslinker featuring a hydrophilic tetraethylene glycol (PEG4) spacer terminated with a DBCO (dibenzocyclooctyne) group for strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. This compound is essential for creating stable, bioorthogonal conjugates under mild, copper-free conditions, which is critical for sensitive biological applications. It is primarily needed by researchers and manufacturers in bioconjugation, pharmaceutical development, and advanced material science for labeling biomolecules, developing antibody-drug conjugates (ADCs), and functionalizing surfaces.

Application

• Bioconjugation and ADC Development: Site-specific conjugation of drugs, dyes, or other payloads to antibodies, proteins, or peptides via DBCO-azide click chemistry.
• Proteomics and Biomarker Research: Labeling and enrichment of azide-tagged biomolecules for mass spectrometry analysis and cellular imaging.
• Polymer and Material Science: Functionalization of nanoparticles, hydrogels, and surfaces with bioactive ligands to create smart materials and drug delivery systems.
• Diagnostic Assay Development: Immobilization of capture molecules on biosensor chips or microarrays for high-throughput screening.
• Chemical Biology Tool Development: Synthesis of probes for studying cellular processes and protein interactions in live cells.
• Oligonucleotide Modification: Conjugation of fluorescent tags or other functional groups to azide-modified DNA or RNA strands.

Basic Information

Product Name	m-Peg4-Dbco
CAS No.	2228857-36-9
Molecular Formula	C31H38N2O7
Molecular Weight	550.65 g/mol
Synonyms	DBCO-PEG4-acid; DBCO-PEG4-COOH; Dibenzocyclooctyne-PEG4-acid; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (2,5,8,11-tetraoxatridecan-13-yl) carbamate; 3,6,9,12-Tetraoxapentadecanoic acid, 15-[(1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-ylmethylcarbamate]; m-PEG4-DBCO; DBCO-acid-PEG4; Azadibenzocyclooctyne-PEG4-acid
EINECS	Contact for details

Quality Control

Our m-Peg4-Dbco is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for critical research and development applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. Allow the vial to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.