Description

n-[5-[[2-(2,5-Dioxo-1-Pyrrolidinyl)Ethyl]Ethylamino]-2-[(4-Nitrophenyl)Azo]Phenyl]Acetamide is a specialized azo dye intermediate featuring a reactive maleimide group, making it a valuable building block for advanced material synthesis. This compound is engineered to enable covalent conjugation to thiol groups, offering a robust and stable linkage for creating functionalized polymers, bioconjugates, and surface coatings. It is primarily utilized by R&D chemists and process engineers in the pharmaceutical, biotechnology, and specialty chemical industries for developing targeted drug delivery systems, diagnostic assays, and high-performance pigments.

Application

• Bioconjugation Reagent: Used for site-specific labeling of proteins, antibodies, and peptides via cysteine residues for diagnostic and therapeutic applications.
• Polymer Functionalization: Serves as a key monomer or cross-linker for synthesizing smart polymers, hydrogels, and surface-modified nanoparticles.
• Diagnostic Assay Development: Employed in the fabrication of enzyme-linked immunosorbent assays (ELISA) and lateral flow tests as a colored or detectable hapten conjugate.
• Material Science Research: Acts as a photoactive or reactive dye for studying polymer dynamics, surface interactions, and creating functional coatings.
• Chromatography & Sensing: Can be used to develop stationary phases for affinity chromatography or as a component in chemical sensors.

Basic Information

Product Name	n-[5-[[2-(2,5-Dioxo-1-Pyrrolidinyl)Ethyl]Ethylamino]-2-[(4-Nitrophenyl)Azo]Phenyl]Acetamide
CAS No.	29649-48-7
Molecular Formula	C22H24N6O5
Molecular Weight	452.47 g/mol
Synonyms	N-[5-[[2-(2,5-Dioxo-1-pyrrolidinyl)ethyl]ethylamino]-2-[(4-nitrophenyl)azo]phenyl]acetamide; Acetamide, N-[5-[[2-(2,5-dioxo-1-pyrrolidinyl)ethyl]ethylamino]-2-[(4-nitrophenyl)azo]phenyl]-; Maleimide-functionalized azo dye; Reactive azo dye intermediate; 4-[[4-[(2-((2,5-Dioxo-1-pyrrolidinyl)ethyl)ethylamino)phenyl]azo]nitrobenzene acetamide; Azo-Maleimide crosslinker
EINECS	Contact for details

Quality Control

Our production of this high-purity intermediate adheres to strict ISO 9001 quality management standards. Every batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure structural identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, impurity profile, and physical properties is provided with each shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed after opening to prevent degradation from atmospheric moisture. Keep away from incompatible materials such as strong bases and reducing agents.

Specification

Item	Specification
Appearance	Orange to red-brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Heavy Metals	≤ 20 ppm
Residual Solvents	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.