Description

4-(4-Dimethylaminophenylazo) β-Lactoside is a specialized synthetic dye intermediate, specifically an azo compound conjugated with a lactoside moiety. This unique structure makes it a valuable chromogenic substrate for enzymatic assays and research in biochemistry and molecular biology. It is primarily utilized by manufacturers and R&D laboratories in the diagnostic, pharmaceutical, and life science sectors for developing sensitive detection systems.

Application

• Chromogenic Substrate for β-Galactosidase: Widely used in molecular biology and microbiology as a colorimetric indicator for the lacZ reporter gene in techniques like blue-white screening.
• Diagnostic Reagent Manufacturing: Serves as a key component in the formulation of diagnostic kits for enzyme-linked immunosorbent assays (ELISA) and other clinical chemistry tests.
• Biochemical Research: Employed in enzymatic activity studies and inhibitor screening assays targeting β-galactosidase and related enzymes.
• Histochemistry and Cell Staining: Used as a staining agent in histological applications to visualize β-galactosidase activity in tissues and cells.
• Food and Environmental Testing: Potential application in microbial detection systems for food safety and environmental monitoring.
• Pharmaceutical Development: Utilized in drug discovery research for high-throughput screening (HTS) platforms.

Basic Information

Product Name	4-(4-Dimethylaminophenylazo) β-Lactoside
CAS No.	27597-77-9
Molecular Formula	C22H28N4O11
Molecular Weight	524.48 g/mol
Synonyms	4-(4-Dimethylaminophenylazo)β-D-galactopyranoside; X-Galactoside (analog); p-(Dimethylamino)azobenzene-β-D-galactopyranoside; Azo-β-galactoside; Galactopyranoside, 4-[(4-dimethylamino)phenyl]azo]-; β-Galactosidase Chromogenic Substrate; LacZ Substrate Analog; 4-Dimethylaminoazobenzene-4'-β-D-galactopyranoside
EINECS	Contact for details

Quality Control

Our 4-(4-Dimethylaminophenylazo) β-Lactoside is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic methods for structural confirmation, to ensure it meets the high standards required for research and diagnostic applications. A detailed Certificate of Analysis (COA) is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed in a dry environment to prevent moisture absorption. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red powder or crystalline solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.