Description

4',5'-Dinitrofluorescein is a chemically modified derivative of fluorescein, characterized by the introduction of nitro groups at the 4' and 5' positions. This modification is crucial for its role as a key synthetic intermediate in the production of advanced fluorescent dyes and biological staining agents. It is primarily utilized by manufacturers and researchers in the life sciences, analytical chemistry, and specialty chemical sectors who require high-purity intermediates for developing sensitive detection and imaging systems.

Application

• Synthesis of Fluorescent Probes and Dyes: Serves as a critical precursor for manufacturing nitro-substituted fluorescein derivatives with tailored spectral properties.
• Biochemical Research and Staining: Used in the development of specific stains and tags for cellular imaging, histology, and fluorescence microscopy applications.
• Analytical Chemistry Standards: Acts as a reference standard or intermediate in the calibration and development of chromatographic and spectroscopic methods.
• Photodynamic Therapy (PDT) Research: Investigated as a potential precursor for photosensitizer compounds used in medical research.
• Sensor Technology Development: Employed in the research and production of chemical sensors and indicators that respond to specific environmental changes.
• Organic Synthesis Building Block: Provides a versatile nitro-aromatic scaffold for further chemical transformations in academic and industrial organic synthesis.

Basic Information

Product Name	4',5'-Dinitrofluorescein
CAS No.	24545-86-6
Molecular Formula	C20H10N2O9
Molecular Weight	422.31 g/mol
Synonyms	4',5'-Dinitrofluorescein; Dinitrofluorescein; 2-(3,4-Dinitro-6-hydroxybenzoyl)benzoic acid; 9-(3,4-Dinitrophenyl)-6-hydroxy-3H-xanthen-3-one; Fluorescein, 4',5'-dinitro-; C.I. 45365; Solvent Yellow 95
EINECS	Contact for details

Quality Control

Our 4',5'-Dinitrofluorescein is manufactured under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment and spectroscopic methods for identity confirmation, to ensure it meets the high standards required for research and synthesis. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting key parameters such as assay purity and impurity profiles.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). Keep away from incompatible materials. Due to its light-sensitive nature, prolonged exposure to direct light should be avoided.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.