Description

Diacetyl 2,4-Dinitrophenylhydrazone is a specialized derivative of 2,4-dinitrophenylhydrazine (DNPH), primarily utilized as a high-purity analytical reference standard and a key reagent in organic synthesis. Its value lies in its role as a critical derivative for the identification and quantification of carbonyl compounds, particularly diacetyl and other α-diketones, via HPLC and spectrophotometric methods. This compound is essential for analytical laboratories, quality control departments, and research institutions in the chemical, pharmaceutical, and food flavoring industries where precise carbonyl analysis is required.

Application

• Analytical Reference Standard: Primary use as a certified reference material (CRM) for calibrating instruments and validating analytical methods for carbonyl compound detection.
• Carbonyl Compound Derivatization: Acts as a derivatizing agent to form stable hydrazone derivatives of diacetyl (2,3-butanedione) and similar α-diketones for enhanced detection in HPLC and UV-Vis analysis.
• Food and Flavor Analysis: Used in the quantification of diacetyl, a key flavor compound, in dairy products (e.g., butter, cheese), fermented beverages, and other foodstuffs to ensure quality and safety.
• Pharmaceutical Impurity Profiling: Employed in the analysis of potential genotoxic impurities (PGIs) and degradation products in active pharmaceutical ingredients (APIs) that contain carbonyl functionalities.
• Environmental Monitoring: Application in monitoring airborne carbonyl compounds (e.g., from industrial emissions) by derivatization onto DNPH-coated sampling cartridges followed by analysis.
• Organic Synthesis Intermediate: Serves as a building block or protecting group in the synthesis of more complex heterocyclic compounds and fine chemicals.
• Research & Development: Fundamental reagent in academic and industrial R&D for studying reaction mechanisms involving carbonyl groups and developing new analytical protocols.

Basic Information

Product Name	Diacetyl 2,4-Dinitrophenylhydrazone
CAS No.	2256-00-0
Molecular Formula	C₁₀H₁₀N₄O₄
Molecular Weight	250.21 g/mol
Synonyms	2,3-Butanedione 2,4-dinitrophenylhydrazone; Diacetyl dinitrophenylhydrazone; Biacetyl 2,4-dinitrophenylhydrazone; 2,4-DNPH derivative of diacetyl; (E)-1-(2,4-Dinitrophenyl)-2-(1-methylidene-2-oxopropyl)hydrazine; Butane-2,3-dione 2,4-dinitrophenylhydrazone; 2,3-Butanedione dinitrophenylhydrazone
EINECS	Contact for details

Quality Control

Our Diacetyl 2,4-Dinitrophenylhydrazone is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistency, meeting the exacting standards required for analytical reference materials. Certificates of Analysis (COA) detailing purity, identity confirmation (IR, NMR), and impurity profiles (HPLC) are provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The compound is light-sensitive; prolonged exposure to light should be avoided to prevent degradation. Keep the container tightly sealed in a dry environment to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.