Description

3H-Indolium, 2-[3-[1,3-Dihydro-3,3-Dimethyl-1-[6-Oxo-6-(2-Propyn-1-Ylamino)Hexyl]-5-Sulfo-2H-Indol-2-Ylidene]-1-Propen-1-Yl]-1-Ethyl-3,3-Dimethyl-5-Sulfo- is a highly specialized, functionalized cyanine dye derivative. This compound matters for its unique photophysical properties, making it a critical intermediate in the development of advanced fluorescent probes and labeling reagents. It is primarily needed by research institutions and manufacturers in the life sciences, diagnostics, and material science sectors for applications requiring near-infrared (NIR) fluorescence and specific molecular conjugation capabilities.

Application

• Fluorescent Labeling Reagents: Key intermediate for synthesizing reactive dyes used to label biomolecules such as proteins, antibodies, and nucleic acids for detection and imaging.
• Bioimaging Probes: Used in the development of NIR fluorescent probes for in vivo and in vitro cellular imaging, offering deeper tissue penetration and reduced background autofluorescence.
• Diagnostic Assays: Serves as a core component in the manufacture of fluorescent conjugates for ELISA, flow cytometry, and lateral flow immunoassays.
• Photodynamic Therapy (PDT) Research: Investigated as a potential photosensitizer precursor due to its light-absorbing characteristics in the NIR region.
• Material Science: Utilized in the synthesis of functional dyes for optical materials, sensors, and non-linear optical applications.
• Chemical Biology: Acts as a building block for creating activity-based probes to study enzyme function and protein interactions.

Basic Information

Product Name	3H-Indolium, 2-[3-[1,3-Dihydro-3,3-Dimethyl-1-[6-Oxo-6-(2-Propyn-1-Ylamino)Hexyl]-5-Sulfo-2H-Indol-2-Ylidene]-1-Propen-1-Yl]-1-Ethyl-3,3-Dimethyl-5-Sulfo-
CAS No.	1010386-62-5
Molecular Formula	C₃₄H₄₄N₄O₆S₂
Molecular Weight	656.86 g/mol
Synonyms	Sulfo-Cyanine5 alkyne; Cy5 Alkyne, sulfonated; 1-Ethyl-2-[3-(1-ethyl-3,3-dimethyl-5-sulfoindolin-2-ylidene)propenyl]-3,3-dimethyl-1-(6-oxo-6-(prop-2-yn-1-ylamino)hexyl)-5-sulfo-3H-indolium; NIR Fluorescent Alkyne Dye; Sulfonated Pentamethine Cyanine Alkyne; Hydrophilic Cy5 Alkyne; Biofunctional Cyanine Dye Intermediate; Contact for details on additional trade names.
EINECS	Contact for details

Quality Control

Our production of this advanced dye intermediate adheres to rigorous quality testing protocols to ensure batch-to-batch consistency and performance. Quality is verified through multiple analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with each shipment to support your research and development traceability requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed under an inert atmosphere or with desiccant after opening to prevent degradation. Keep away from incompatible materials.

Specification

Item	Specification
Appearance	Dark green to blue-black solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Absorption λmax	646 nm ± 5 nm
Emission λmax	662 nm ± 5 nm
Single Spot (TLC)	One major spot

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.