Description

(T-4)-[n-[6-[(2,5-Dioxo-1-Pyrrolidinyl)Oxy]-6-Oxohexyl]-2-[4-[5-[[5-(2-Thienyl)-2H-Pyrrol-2-Ylidene-κn]Methyl]-1H-Pyrrol-2-Yl-κn]Phenoxy]Acetamidato]Difluoroboron is a sophisticated organoboron complex featuring a BODIPY (boron-dipyrromethene) core functionalized with an N-hydroxysuccinimide (NHS) ester linker. This compound matters as a highly reactive and versatile fluorescent labeling reagent, enabling the covalent tagging of biomolecules for advanced detection and imaging applications. It is primarily needed by researchers and manufacturers in the life sciences, diagnostics, and pharmaceutical development sectors for creating sensitive probes and assays.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to primary amines on proteins, antibodies, peptides, and oligonucleotides via its NHS ester group for fluorescence detection.
• Bioimaging Probe Development: Synthesis of targeted fluorescent probes for cellular and tissue imaging in microscopy (e.g., confocal, fluorescence).
• Diagnostic Assay Reagent: Used in the development of fluorescent immunoassays (FLISA), flow cytometry, and other diagnostic platforms requiring high-sensitivity detection.
• Chemical Biology Research: A tool for studying biomolecular interactions, localization, and dynamics in live or fixed cells.
• Sensor Fabrication: Incorporation into polymeric or solid-state matrices for creating chemical or biological sensors.
• Pharmaceutical Analysis: As a derivatization agent for the HPLC or LC-MS analysis of amine-containing drugs or metabolites.

Basic Information

Product Name	(T-4)-[n-[6-[(2,5-Dioxo-1-Pyrrolidinyl)Oxy]-6-Oxohexyl]-2-[4-[5-[[5-(2-Thienyl)-2H-Pyrrol-2-Ylidene-κn]Methyl]-1H-Pyrrol-2-Yl-κn]Phenoxy]Acetamidato]Difluoroboron
CAS No.	197306-80-2
Molecular Formula	C₃₁H₂₈BF₂N₃O₆S
Molecular Weight	619.44 g/mol
Synonyms	BODIPY FL NHS Ester; BODIPY® FL NHS Ester (Thienyl derivative); 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-indacene-3-Propionic Acid, NHS Ester (Thienyl analog); BDP FL NHS Ester; BODIPY-FL, SE; 6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl 2-(4-(5-((5-(thiophen-2-yl)-1H-pyrrol-2-yl)methylene)-4,4-difluoro-4-bora-3a,4a-diaza-s-indacen-3-yl)phenoxy)acetate
EINECS	Contact for details

Quality Control

Our BODIPY FL NHS Ester is produced under strict quality protocols to ensure batch-to-batch consistency and performance reliability for critical research and diagnostic applications. Each lot undergoes comprehensive analytical characterization, including HPLC purity analysis, spectroscopic identity confirmation (NMR, MS), and functional performance testing. A detailed Certificate of Analysis (COA) documenting purity, absorbance/fluorescence properties, and other key specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize condensation and hydrolysis of the active NHS ester group.

Specification

Item	Specification
Appearance	Orange to red solid or film
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular ion [M]⁺
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	~503 - 508 nm
Emission Maximum	~510 - 515 nm
Extinction Coefficient (ε)	Contact for details
Fluorescence Quantum Yield	Contact for details
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.