Description

5-(Bromomethyl)Fluorescein is a key fluorescent labeling reagent and versatile chemical building block. Its core value lies in its reactive bromomethyl group, which facilitates covalent conjugation to biomolecules like proteins and peptides, enabling sensitive detection and analysis. This compound is essential for researchers and manufacturers in the life sciences, particularly for developing diagnostic assays, fluorescent probes, and advanced materials. Its application extends to areas requiring high-sensitivity fluorescence tagging and molecular imaging.

Application

• Fluorescent Labeling Reagent: Primary use for covalently tagging proteins, antibodies, peptides, and other biomolecules via its reactive bromomethyl group.
• Diagnostic Assay Development: Critical component in the synthesis of fluorescent conjugates for ELISA, lateral flow assays, and other immunodiagnostic platforms.
• Bioconjugation & Probe Synthesis: Serves as a foundational intermediate for creating custom fluorescent probes, sensors, and molecular beacons for cellular imaging.
• Life Science Research: Used in fluorescence microscopy, flow cytometry (FACS), and immunohistochemistry (IHC) for visualizing and quantifying biological targets.
• Material Science: Potential use in the development of fluorescent polymers, smart materials, and sensors where a fluorescein derivative is required.
• Pharmaceutical Analysis: Can be employed as a derivatization agent in HPLC or capillary electrophoresis for detecting trace-level analytes.

Basic Information

Product Name	5-(Bromomethyl)Fluorescein
CAS No.	148942-72-7
Molecular Formula	C21H13BrO5
Molecular Weight	425.23 g/mol
Synonyms	5-(Bromomethyl)fluorescein; Fluorescein, 5-(bromomethyl)-; 5-Bromomethylfluorescein; 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-5-(bromomethyl)benzoic Acid; 5-BMF; BMF; 5-Bromomethyl fluorescein; 9-(2-Carboxy-4-(bromomethyl)phenyl)-6-hydroxy-3H-xanthen-3-one
EINECS	Contact for details

Quality Control

Our 5-(Bromomethyl)Fluorescein is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant quality management standards to support our global customer base in pharmaceutical, diagnostic, and research sectors.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed under desiccant and away from direct light exposure to maintain stability and purity.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Loss on Drying	≤ 2.0 %
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in alcohols

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.