Description

Sulfosuccinimidyl-n-(3-(Acetylthio)-3-Methylbutyryl)-β-Alanine is a specialized heterobifunctional crosslinking reagent designed for advanced bioconjugation. This compound is engineered to facilitate the stable and site-specific coupling of biomolecules, such as proteins and peptides, through its amine-reactive NHS ester and thiol-reactive acetylthio groups. It is an essential tool for researchers and manufacturers in the biopharmaceutical, diagnostic, and life science research sectors who require precise and efficient conjugation strategies for developing therapeutics, assays, and novel biomaterials.

Application

• Antibody-Drug Conjugate (ADC) Development: For the site-specific conjugation of cytotoxic payloads to monoclonal antibodies, enabling the creation of targeted cancer therapies.
• Protein and Peptide Labeling: Used to attach fluorescent dyes, enzymes, or other reporter molecules to proteins for diagnostic assays and imaging applications.
• Surface Immobilization: Functionalizing biosensor chips, microarrays, or nanoparticle surfaces with biomolecules for analytical and diagnostic platforms.
• Polymer-Protein Conjugation: Creating PEGylated proteins or other polymer-biomolecule hybrids to improve pharmacokinetic properties.
• Crosslinking for Structural Studies: Investigating protein-protein interactions or stabilizing protein complexes for structural biology.
• Vaccine Development: Conjugating antigens to carrier proteins to enhance immunogenicity.

Basic Information

Product Name	Sulfosuccinimidyl-n-(3-(Acetylthio)-3-Methylbutyryl)-β-Alanine
CAS No.	131068-49-0
Molecular Formula	C14H19N2O8S2
Molecular Weight	407.44 g/mol
Synonyms	N-(3-(Acetylthio)-3-methylbutyryl)-β-alanine N-hydroxysuccinimide ester; 3-[[3-(Acetylthio)-3-methyl-1-oxobutyl]amino]propanoic acid 2,5-dioxo-1-pyrrolidinyl ester; SMPT Crosslinker; SMPT; Heterobifunctional Crosslinker SMPT; N-Succinimidyl 3-((3-(acetylthio)-3-methylbutanoyl)amino)propanoate; β-Alanine, N-[3-(acetylthio)-3-methyl-1-oxobutyl]-, 2,5-dioxo-1-pyrrolidinyl ester
EINECS	Contact for details

Quality Control

Our Sulfosuccinimidyl-n-(3-(Acetylthio)-3-Methylbutyryl)-β-Alanine is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for sensitive research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant quality guidelines to support our customers in GMP-compliant and cGMP environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or crystalline solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.