Description

6-([4,6-Dichlorotriazin-2-Yl]Amino)Fluorescein Hydrochloride is a high-purity, reactive fluorescent labeling reagent designed for covalent conjugation to biomolecules. This compound matters for researchers and manufacturers requiring precise, stable fluorescent tagging of proteins, nucleic acids, and other substrates for detection and analysis. It is essential for industries and research institutions focused on diagnostics, bioanalytical assay development, and life science research, where reliable signal generation is critical.

Application

• Fluorescent Labeling of Proteins and Antibodies: Used as an amine-reactive probe (via the dichlorotriazine group) for preparing fluorescent conjugates in immunoassays (ELISA, Western Blot) and flow cytometry.
• Nucleic Acid Labeling: Employed in the preparation of fluorescently tagged oligonucleotides for fluorescence in situ hybridization (FISH) and microarray applications.
• Bioconjugation and Probe Synthesis: A key intermediate for synthesizing custom fluorescent probes and sensors targeting specific biological structures.
• Diagnostic Kit Manufacturing: Integrated into the production of clinical diagnostic reagents and kits that rely on fluorescent detection.
• Cell and Tissue Staining: Facilitates the development of staining protocols for microscopy and histology to visualize cellular components.
• Biomolecular Interaction Studies: Used in fluorescence polarization (FP) and other biophysical techniques to study binding events.

Basic Information

Product Name	6-([4,6-Dichlorotriazin-2-Yl]Amino)Fluorescein Hydrochloride
CAS No.	118357-32-7
Molecular Formula	C22H12Cl3N5O5 • HCl
Molecular Weight	584.74 g/mol (Free base: 548.72)
Synonyms	6-DTAF Hydrochloride; 6-(4,6-Dichloro-1,3,5-triazin-2-ylamino)fluorescein hydrochloride; DTAF; 6-Fluoresceinisothiocyanate analogue; 6-Isothiocyanatofluorescein analogue; Reactive Fluorescein Derivative; [4,6-Dichloro-s-triazin-2-yl]aminofluorescein hydrochloride
EINECS	Contact for details

Quality Control

Our 6-([4,6-Dichlorotriazin-2-Yl]Amino)Fluorescein Hydrochloride is manufactured under strict quality systems. Every batch undergoes rigorous analytical testing to ensure identity, purity, and performance consistency suitable for sensitive life science applications. A comprehensive Certificate of Analysis (COA) detailing purity (HPLC), spectral data, and functional performance is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label. This product is hygroscopic (moisture-sensitive) and light-sensitive; desiccants are recommended for long-term storage. Keep container tightly sealed under an inert atmosphere when not in use to minimize degradation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	490 - 500 nm
Emission Maximum	510 - 520 nm
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.