Description

n-Hydroxysulfosuccinimide Sodium Salt is a water-soluble, amine-reactive chemical crosslinker widely used in bioconjugation and protein chemistry. Its primary value lies in its ability to efficiently activate carboxyl groups for coupling with primary amines under mild, aqueous conditions, forming stable amide bonds. This makes it an essential reagent for researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic industries who require reliable and efficient bioconjugation strategies.

Application

• Peptide and Protein Conjugation: A key reagent for crosslinking peptides, antibodies, enzymes, and other proteins to carriers, surfaces, or other biomolecules.
• Immunoassay Development: Used in the preparation of enzyme-labeled antibodies and antigens for ELISA and other diagnostic test kits.
• Biomaterial and Hydrogel Fabrication: Facilitates the covalent crosslinking of polymers containing carboxyl groups for creating stable 3D scaffolds in tissue engineering.
• Drug Delivery Systems: Employed to conjugate therapeutic agents (e.g., cytotoxic drugs, peptides) to targeting molecules like antibodies for antibody-drug conjugate (ADC) research.
• Surface Immobilization: Activates carboxylated surfaces (e.g., sensor chips, magnetic beads) for the covalent attachment of biomolecules in biosensor and affinity purification applications.
• Carbodiimide (EDC) Coupling Reactions: Commonly used as a co-reagent with EDC to enhance coupling efficiency and yield by forming a more stable, reactive intermediate.

Basic Information

Product Name	n-Hydroxysulfosuccinimide Sodium Salt
CAS No.	106627-54-7
Molecular Formula	C4H4NNaO7S
Molecular Weight	241.13 g/mol
Synonyms	Sulfo-NHS; N-Hydroxysulfosuccinimide Sodium Salt; Sulfosuccinimidyl Hydroxide Sodium Salt; 1-(3-Sulfopropyl)-3-hydroxy-2,5-pyrrolidinedione Sodium Salt; Sulfo-N-hydroxysuccinimide Sodium Salt; NHS-Sulfonate Sodium Salt; Hydroxy-3-sulfopropyl-2,5-dioxopyrrolidine Sodium Salt; Water-soluble NHS
EINECS	Contact for details

Quality Control

Our n-Hydroxysulfosuccinimide Sodium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and ICP-MS for heavy metal screening, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Heavy Metals (as Pb)	≤ 10 ppm
Solubility (in Water)	Clear, colorless solution
pH (1% Solution)	6.0 - 8.0

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.