Description

6-Carboxy-4',5'-Dimethylfluorescein is a high-purity, functionalized derivative of fluorescein, a cornerstone fluorophore in life science research. This compound is valued for its role as a versatile reactive intermediate in the synthesis of advanced fluorescent probes, dyes, and labeling reagents. It is primarily utilized by researchers and manufacturers in the fields of bioconjugation, diagnostics, and analytical chemistry to develop sensitive detection systems for biomolecules.

Application

• Fluorescent Probe Synthesis: A key precursor for creating custom fluorescent labels and tags for proteins, antibodies, and nucleic acids.
• Bioconjugation Chemistry: The carboxylic acid group enables covalent coupling to amines, facilitating the labeling of biomolecules for imaging and assay development.
• Diagnostic Assay Development: Used in the manufacture of reagents for fluorescence-based immunoassays (e.g., ELISA, lateral flow tests) and in vitro diagnostics (IVD).
• Cell and Tissue Staining: Can be derivatized into dyes for fluorescence microscopy and histology applications.
• Sensor Fabrication: Serves as a building block for developing chemical sensors and indicators for pH, metal ions, or enzymatic activity.
• Research & Development: An essential tool in academic and industrial R&D for exploring new detection methodologies and bioimaging techniques.

Basic Information

Product Name	6-Carboxy-4',5'-Dimethylfluorescein
CAS No.	100111-03-3
Molecular Formula	C25H18O7
Molecular Weight	430.41 g/mol
Synonyms	4',5'-Dimethylfluorescein-6-carboxylic acid; 6-Carboxy-4',5'-dimethylfluorescein; 2-(6-Carboxy-3-oxo-3H-xanthen-9-yl)-4,5-dimethylbenzoic acid; 9-(3-Carboxy-4,5-dimethylphenyl)-6-carboxy-3H-xanthen-3-one; DMFCA; 4',5'-Dimethyl-6-carboxyfluorescein
EINECS	Contact for details

Quality Control

Our 6-Carboxy-4',5'-Dimethylfluorescein is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic identification (NMR, IR), to ensure it meets the stringent requirements for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). Keep the container sealed to protect from moisture.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Loss on Drying	≤ 2.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.