Description

Naphthol As-An Phosphate CAS NO 18228-15-4 is a specialized biochemical reagent primarily used as a substrate for the detection of alkaline phosphatase activity. This compound is critical for research and diagnostic applications where precise enzymatic activity measurement is required. It is essential for professionals in life sciences, clinical diagnostics, and pharmaceutical development who require reliable and high-purity substrates for colorimetric and fluorometric assays.

Application

• Primary substrate in enzymatic assays for alkaline phosphatase (ALP) detection.
• Key reagent in immunohistochemistry (IHC) and enzyme-linked immunosorbent assays (ELISA) for signal generation.
• Used in molecular biology for nucleic acid hybridization techniques like Southern and Northern blotting.
• Essential in clinical diagnostics for detecting ALP isoenzymes in serum.
• Employed in histochemical staining to localize phosphatase activity in tissue sections.
• Component in research kits for studying cell signaling pathways and bone metabolism.
• Used in the development of biosensors and diagnostic strips.
• Applied in pharmaceutical high-throughput screening (HTS) for drug discovery.

Basic Information

Product Name	Naphthol As-An Phosphate
CAS No.	18228-15-4
Molecular Formula	C20H16NO6P
Molecular Weight	397.32 g/mol
Synonyms	Naphthol AS-AN Phosphate; Naphthol AS-AN Phosphate Disodium Salt; 3-Hydroxy-2-naphthoic acid 2,4-dimethylanilide phosphate; 3-Hydroxy-4'-methyl-2-naphthanilide phosphate; Naphthol AS-AN phosphoric acid ester; Fast Red TR Salt substrate; Alkaline Phosphatase Substrate; AS-AN-PO4; Phosphoric acid mono-(4'-methyl-2'-oxo-2'H-[1,3']binaphthalenyl-1'-yl) ester
EINECS	Contact for details

Quality Control

Our Naphthol As-An Phosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided to guarantee the product meets the stringent requirements for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	Off-white to light beige powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Heavy Metals	≤ 20 ppm
Residual Solvents	Meets ICH guidelines
Functional Activity	Suitable for enzyme assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.