Description

5-Nitrofluorescein Diacetate is a chemically modified fluorescein derivative, primarily serving as a key intermediate in the synthesis of advanced fluorescent probes and dyes. Its value lies in the nitro group, which can be selectively reduced or further functionalized, enabling the creation of highly specific labeling reagents for biological and chemical detection. This compound is essential for researchers and manufacturers in the life sciences, diagnostic reagent development, and analytical chemistry sectors who require precise molecular tools for imaging and assay development.

Application

• Fluorescent Probe Synthesis: A critical precursor for developing nitroreductase-activated fluorescent turn-on probes used in hypoxia imaging and cellular activity sensing.
• Diagnostic Reagent Manufacturing: Used in the production of enzyme substrates and labeling compounds for clinical diagnostic kits and immunoassays.
• Bioconjugation & Labeling: Serves as an intermediate for creating fluorescent tags that conjugate to antibodies, proteins, or nucleic acids for microscopy and flow cytometry.
• Chemical Sensing Development: Employed in the research and development of chemosensors for detecting specific ions or small molecules in analytical systems.
• Dye & Pigment Intermediate: Acts as a building block for synthesizing specialized xanthene dyes with tailored photophysical properties.
• Pharmaceutical Research: Utilized in drug discovery as a tracer or a component in developing theranostic agents.

Basic Information

Product Name	5-Nitrofluorescein Diacetate
CAS No.	14926-29-5
Molecular Formula	C24H15NO9
Molecular Weight	461.38 g/mol
Synonyms	5-Nitrofluorescein diacetate; Fluorescein, 5-nitro-, diacetate; 2',7'-Bis(acetyloxy)-5-nitro-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one; 5-Nitrofluorescein acetate; 5-Nitro-2',7'-dichlorofluorescein diacetate (common alias); 5-NF-DA; 5-Nitro-FDA
EINECS	Contact for details

Quality Control

Our 5-Nitrofluorescein Diacetate is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing to ensure high purity and consistency, meeting the stringent requirements for research and diagnostic applications. A Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to guarantee traceability and compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is light-sensitive (store away from light) and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Yellow to orange-yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Melting Point	Contact for details
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.