Description

Ethylenediaminetetraacetic Acid Dilithium Salt is a high-purity, water-soluble chelating agent widely used in biochemical and pharmaceutical research. Its primary value lies in its ability to effectively sequester di- and trivalent metal ions, such as calcium and magnesium, under neutral to slightly alkaline conditions. This makes it an essential reagent for molecular biologists, diagnostic kit manufacturers, and pharmaceutical formulators who require precise control of metal ion concentrations in sensitive enzymatic and buffer systems.

Application

• Molecular Biology & Biochemistry: A key component in lysis, binding, and wash buffers for nucleic acid purification kits (e.g., plasmid DNA, RNA) to inhibit metal-dependent nucleases.
• Diagnostic Reagent Formulation: Used as a stabilizer and anticoagulant in clinical chemistry reagents and blood collection tubes.
• Pharmaceuticals: Employed as a stabilizing agent in injectable formulations and ophthalmic solutions to chelate trace metals that catalyze degradation.
• Cell Culture & Tissue Engineering: Incorporated into cell dissociation solutions (e.g., Versene) to facilitate gentle cell detachment without the use of proteolytic enzymes.
• Enzyme Activity Studies: Serves as a specific inhibitor of metalloenzymes in research applications to elucidate enzymatic mechanisms.
• Industrial Process Chemistry: Acts as a sequestrant in specific catalytic processes or to prevent metal-ion catalyzed side reactions.

Basic Information

Product Name	Ethylenediaminetetraacetic Acid Dilithium Salt
CAS No.	14531-56-7
Molecular Formula	C10H14Li2N2O8 • xH2O
Molecular Weight	Anhydrous: 254.10 g/mol
Synonyms	EDTA Dilithium Salt; Dilithium EDTA; Ethylenedinitrilotetraacetic Acid Dilithium Salt; Dilithium Edetate; Edetate Dilithium; Dilithium Ethylenediaminetetraacetate; N,N'-1,2-ethanediylbis[N-(carboxymethyl)glycine] dilithium salt; Li2EDTA
EINECS	238-572-7

Quality Control

Our Ethylenediaminetetraacetic Acid Dilithium Salt is manufactured under a strict quality management system. Each batch is tested to ensure it meets high-purity standards suitable for research and in-vitro diagnostic use. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing parameters such as assay, purity, heavy metal content, and loss on drying. We can supply material compliant with relevant sections of USP/NF or other pharmacopeial monographs upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed after each use to prevent moisture absorption, which can affect weight and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms
Assay (Anhydrous Basis)	98.0% - 102.0%
pH (1% Solution)	7.0 - 9.0
Loss on Drying	≤ 5.0%
Heavy Metals (as Pb)	≤ 20 ppm
Chloride (Cl)	≤ 0.05%
Sulfate (SO4)	≤ 0.05%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.