Description

Diformazan Dye CAS NO 12797-93-2 is a specialized chromogenic compound widely used as a critical reagent in biochemical and analytical applications. Its primary value lies in its ability to form intensely colored formazan products, serving as a sensitive indicator for various enzymatic and cellular assays. This product is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic industries who require reliable and high-purity detection reagents.

Application

• Cell Viability and Proliferation Assays: Used as a substrate in MTT and related assays to measure mitochondrial activity in live cells.
• Diagnostic Kits: Serves as a key colorimetric indicator in point-of-care and laboratory diagnostic test strips and kits.
• Enzyme-Linked Immunosorbent Assay (ELISA): Acts as a chromogenic substrate for horseradish peroxidase (HRP) and other enzymes in immunoassays.
• Microbiology Research: Employed in assays to study bacterial viability and metabolic activity.
• Biochemical Research: Utilized as a redox indicator in various enzymatic reaction studies and high-throughput screening.
• Histochemistry and Cytochemistry: Applied in staining protocols to localize enzyme activity in tissue and cell samples.

Basic Information

Product Name	Diformazan Dye
CAS No.	12797-93-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Diformazan; Tetrazolium Dye Derivative; Formazan Dye; MTT Formazan; 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide formazan; Thiazolyl Blue Formazan; Tetrazolium Salt Reduction Product; Nitro Blue Tetrazolium (NBT) Formazan
EINECS	Contact for details

Quality Control

Our Diformazan Dye is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including purity assessment by HPLC and spectroscopic identification, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	Dark colored powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Loss on Drying	≤ 2.0 %
Residue on Ignition	≤ 1.0 %
Spectral Characteristics	Conforms to standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.